The Norwegian Mother And Child Study: Specimen Collectio

目的：创建一个生物标本库，以评估环境暴露和对孕妇及其未出生的孩子的健康影响。我们预计在3-4年内招收50,000名女性及其后代。程序和技术：将在怀孕第17周从妇女身上采集全血、血浆和尿液样本。正在实施严格的质量保证和质量控制计划，以确保样品质量，防止潜在的环境污染。每位女性的样本将包括1x2ml用于微量金属分析的EDTA真空容器，储存在-20oC的全血，~3ml从肝素管储存在-80oC的血浆，以及10ml的尿液，储存在-80oC。这一资源将与母婴队列研究中有关妇女和儿童的更多数据相联系。成就：制定了样本收集和储存议定书，并得到各方的同意。样本采集预计将于2001年秋季开始。

Aims: Create a biological specimen repository to evaluate environmental exposures and health effects to pregnant women and their unborn children. We anticipate enrolling 50,000 women and their offspring over 3-4 years. Procedures and techniques: Whole blood, plasma and urine samples will be collected from women at the 17th week of gestation. A rigorous quality assurance and quality control plan is being implemented to ensure sample quality with regard to potential environmental contamination. Samples from each woman will include 1x2ml EDTA vacutainer of whole blood for trace metal analysis stored at -20oC, ~3 ml of plasma from a heparin tube stored at -80oC, and 10 ml of urine stored at -80oC. This resource will be linked to additional data on the women and children in the mother and child cohort study. Accomplishments: The sample collection and storage protocol has been developed and agreed upon by all parties. Sample collection is anticipated to start Fall 2001.