The Norwegian Mother And Child Study: Environmental Spec

我的研究重点是通过改进暴露测量来评估常见环境暴露对健康的影响。为了创造资源以加强接触测量，我参与了在挪威一个大的出生队列的背景下创建生物标本库的工作，以创建一个生物标本库，以评估环境暴露和对孕妇及其未出生的孩子的健康影响。我们预计在3-4年内招收50,000名女性及其后代。 我们目前正在进行试点工作，以评估防止尿样细菌污染的方法，同时仍保留未来感兴趣的分析物(酚、邻苯二甲酸盐、非持久性农药、砷、汞)。与挪威调查人员合作，我们正在设计一项质量保证实验和方案，以评估各种参数的储存条件。这将需要大约2年的时间才能完成。我们还在设计一项研究，使用NIEHS样本并链接到母亲的问卷数据和医学出生登记数据，以评估一种常见环境污染物--可能是邻苯二甲酸盐--对健康的影响。 将在怀孕第17周采集孕妇的全血、血浆和尿液样本。正在实施严格的质量保证和质量控制计划，以确保样品质量，防止潜在的环境污染。每位女性的样本将包括1x2ml用于微量金属分析的EDTA真空容器，储存在-20oC的全血，~3ml从肝素管储存在-80oC的血浆，以及10ml的尿液，储存在-80oC。这一资源将与挪威母婴队列研究中有关妇女和儿童的其他数据相联系。 样本收集和储存议定书已经制定，并得到各方的同意。IRB已收到参加NIEHS的批准，并已开始样本采集。

My research focuses on assessing health effects of common environmental exposures through improved exposure measurement. In an effort to create resources to enhance exposure measurement, I have been involved in the creation of a biological specimen repository in the context of a large birth cohort in Norway to create a biological specimen repository to evaluate environmental exposures and health effects to pregnant women and their unborn children. We anticipate enrolling 50,000 women and their offspring over 3-4 years. We are currently conducting pilot work to assess means to prevent bacterial contamination of urine samples while still maintaining the analytes of future interest (phenols, phthalates, non-persistent pesticides, arsenic, mercury). In collaboration with Norwegian investigators, we are designing a quality assurance experiment and protocol to assess storage conditions for a variety of parameters. This will take ~2 years to complete. We are also designing a study to use the NIEHS samples and link to the questionnaire data from the mother and the Medical Birth Registry data to assess health effects of a common environmental contaminant, probably phthalates. Whole blood, plasma and urine samples will be collected from women at the 17th week of gestation. A rigorous quality assurance and quality control plan is being implemented to ensure sample quality with regard to potential environmental contamination. Samples from each woman will include 1x2ml EDTA vacutainer of whole blood for trace metal analysis stored at -20oC, ~3 ml of plasma from a heparin tube stored at -80oC, and 10 ml of urine stored at -80oC. This resource will be linked to additional data on the women and children in the Norway Mother and Child cohort study. The sample collection and storage protocol has been developed and agreed upon by all parties. IRB approval has been received for NIEHS participation and sample collection has begun.