Type 1 Collagen-Based Nerve Guide for PNS Regeneration

该提案是周围神经修复和再生多学科领域的合作，涉及胶原蛋白基质公司，领导BRP，专注于细胞外基质设计和工程;东弗吉尼亚医学院，专注于神经科学和神经外科;杜克大学，专注于神经科学，周围神经的神经修复，临床神经病学和泌尿学;和大学。 南佛罗里达，专攻临床泌尿学。这项研究合作计划的总体目标是设计、设计和评估用于周围神经再生应用的I型胶原基生物活性神经引导物。研究团队通过合作伙伴的共同努力，应用当前最先进的基质技术，蛋白质化学，神经科学，神经外科和临床泌尿学知识，系统地评估关键设计参数。这种系统的方法将导致神经引导的发展，在神经再生的临床成功的可能性很高。该提案的具体目标涉及关键设计参数的隔离，并在大鼠坐骨神经模型中对其进行测试。根据最佳设计参数设计的最终原型将在两种灵长类动物神经模型（正中神经和海绵体神经）中进行评估，作为临床应用的潜在神经修复方法。在第一年，我们将隔离设计参数并设计原型。在随后的几年中，我们将对原型进行表征，在大鼠模型中测试原型的体内性能，最终确定整体设计，最后在灵长类动物模型中评估最终原型。整个计划为期五年。

This proposal is a collaboration of multidisciplinary fields of peripheral nerve repair and regeneration, involving Collagen Matrix, Inc., leading the BRP, specializing in extracellular matrix design and engineering; Eastern Virginia Medical School, specializing in neuroscience and neurosurgery; Duke University specializing in neuroscience, entubulation repair of peripheral nerve, clinical neurology and urology; and University of . South Florida, specializing in clinical urology. The overall goal of this research partnership proposal is to design, engineer and evaluate in vivo a type I collagen-based bioactive nerve guide for peripheral nerve regeneration applications. The research team, through joint effort of the partnership, applies the current state-of-the- art knowledge of matrix technology, protein chemistry, neuroscience, neurosurgery and clinical urology to systematically evaluate the key design parameters. This systematic approach will lead to the development of a nerve guide that has the high probability of clinical success in nerve regeneration. The specific objectives of the proposal involve the isolation of the key design parameters and testing them in a rat sciatic nerve model. The final prototype, engineered from optimal design parameters, will be evaluated in two primate nerve models (median and cavernosal) as a potential entubulation repair method for clinical application. In the first year we will isolate the design parameters and engineer the prototypes. In subsequent years, we will characterize the prototypes, test in vivo performance of the prototypes in a rat model, finalize the overall design, and finally, evaluate the final prototype in primate models. The overall program is designed for five years.