METAL BINDING SITES ON CELL WALLS OF DATURA INNOXIA CULTURE CELLS SUSPENSION

曼陀罗培养细胞悬浮液细胞壁上的金属结合位点

• 批准号: 6586799
• 负责人: JANE G DEWITT
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586799
• 关键词: biomaterials; biomedical resource; environmental toxicology; plants; proteins; spectrometry; structural biology

Metal-tolerant plants function by excluding or reducing the uptake of the toxic ion, accumulating the metal in cell walls, or complexing the metal in some way to decrease its toxicity. Datura innoxia (Jimsonweed) is a cadmium-tolerant plant that synthesizes polypeptides to sequester cadmium internally in the cell and chelates heavy metal ions externally on the cell wall. The nature of the metal binding sites on the cell walls is not known, however, there are a number of functional groups derived from cell wall proteins, lipids and carbohydrates capable of coordinating metal ions. Our goal is to use X-ray absorption spectroscopy to directly probe the metal binding sites on D. innoxia cell walls, specifically Ba2+, Cu2+ and Cd2+ metal binding sites. Understanding the interaction between plant cell walls and heavy metal ions is important because metal accumulation in agriculturally important plants is the principle means by which heavy metals are introduced into the food web. In addition, removal of toxic metals from the environment by plants or plant products has potential as an effective means of remediating heavy metal and radionuclide waste.

耐金属植物的功能是排除或减少吸收 在细胞壁中积累金属，或络合 以某种方式降低其毒性。 白花曼陀罗 曼陀罗（Jimsonweed）是一种耐镉植物， 将镉隔离在细胞内部并螯合重金属 细胞壁上的离子。 金属结合的性质 细胞壁上的位点是未知的，然而，有许多 衍生自细胞壁蛋白质、脂质和 能够配位金属离子的碳水化合物。 我们的目标是利用 X射线吸收光谱法直接探测金属结合 网站D.无氧细胞壁，特别是Ba2+、Cu2+和Cd2+金属 结合位点。 了解植物细胞壁之间的相互作用 和重金属离子是重要的，因为金属积累在 农业上重要的植物是主要手段， 金属被引入食物网。 此外，移除 植物或植物产品从环境中吸收有毒金属， 潜在的作为一种有效的手段修复重金属和 放射性核素废物。