XAS INVESTIGATION OF HEAVY METAL TRANSFORMATION IN PLANTS

在快速周转下进行的初始X射线吸收研究 XAS方案1E23表明XAS对不同的Cd敏感 细胞壁和金属结合多肽中的环境 无毒曼陀罗耐金属性组织培养。这些研究表明 有可能使用XAS来跟踪现场 重金属积累植物对重金属的转化作用。基于 这些结果，我们建议扩展这项工作，以表征镍 和铬在金黄色葡萄球菌细胞壁和分离的金属络合物上的位置 来自白花蛇舌兰的植物细胞。我们还建议使用XAS来遵循 白花蛇舌草组织培养对Cd的原位转化。这些 研究将通过以下方式提供对金属离子转化的洞察 在细胞水平上耐金属的植物，并有助于 对生物转化途径的理解 金属离子在环境中的命运。

Initial x-ray absorption studies performed under Rapid Turnaround XAS proposal 1E23 showed that XAS is sensitive to the different Cd environments on the cell wall and in a metal-binding polypeptide from metal-tolerant Datura innoxia tissue culture. These studies suggest that it may be possible to use XAS to follow the in situ transformation of heavy metals by metal-accumulating plants. Based on these results, we propose to extend this work to characterize the Ni and Cr sites on D. innoxia cell walls and in metal complexes isolated from D. innoxia plant cells. We also propose to use XAS to follow the in situ transformation of Cd by D. innoxia tissue culture. These studies will provide insight into the transformation of metal ions by metal-tolerant plants on a cellular level, and contribute to an understanding of the biotic transformation pathways that contribute to the fate of metal ions in the environment.