XAS INVESTIGATION OF HEAVY METAL TRANSFORMATION IN PLANTS

在快速周转下进行的初始X射线吸收研究 XAS提案1E23表明，XAS对不同的Cd敏感 在细胞壁上的环境和金属结合多肽中， 耐重金属曼陀罗组织培养 这些研究表明 这可能是可能的，使用XAS跟踪原位 重金属富集植物对重金属的转化。 基于 这些结果，我们建议扩展这项工作，以表征镍 和Cr位点。无氧细胞壁和金属络合物分离 来自D.无害植物细胞。 我们还建议使用XAS来遵循 Cd在D.无氧组织培养 这些 研究将提供对金属离子转化的深入了解， 金属耐受植物在细胞水平上，并有助于 了解生物转化途径，有助于 金属离子在环境中的命运

Initial x-ray absorption studies performed under Rapid Turnaround XAS proposal 1E23 showed that XAS is sensitive to the different Cd environments on the cell wall and in a metal-binding polypeptide from metal-tolerant Datura innoxia tissue culture. These studies suggest that it may be possible to use XAS to follow the in situ transformation of heavy metals by metal-accumulating plants. Based on these results, we propose to extend this work to characterize the Ni and Cr sites on D. innoxia cell walls and in metal complexes isolated from D. innoxia plant cells. We also propose to use XAS to follow the in situ transformation of Cd by D. innoxia tissue culture. These studies will provide insight into the transformation of metal ions by metal-tolerant plants on a cellular level, and contribute to an understanding of the biotic transformation pathways that contribute to the fate of metal ions in the environment.