METAL BINDING SITES ON CELL WALLS OF DATURA INNOXIA CULTURE CELLS SUSPENSION

曼陀罗培养细胞悬浮液细胞壁上的金属结合位点

• 批准号: 6658766
• 负责人: JANE G DEWITT
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658766
• 关键词: biomaterials; biomedical resource; environmental toxicology; plants; proteins; spectrometry; structural biology

Metal-tolerant plants function by excluding or reducing the uptake of the toxic ion, accumulating the metal in cell walls, or complexing the metal in some way to decrease its toxicity. Datura innoxia (Jimsonweed) is a cadmium-tolerant plant that synthesizes polypeptides to sequester cadmium internally in the cell and chelates heavy metal ions externally on the cell wall. The nature of the metal binding sites on the cell walls is not known, however, there are a number of functional groups derived from cell wall proteins, lipids and carbohydrates capable of coordinating metal ions. Our goal is to use X-ray absorption spectroscopy to directly probe the metal binding sites on D. innoxia cell walls, specifically Ba2+, Cu2+ and Cd2+ metal binding sites. Understanding the interaction between plant cell walls and heavy metal ions is important because metal accumulation in agriculturally important plants is the principle means by which heavy metals are introduced into the food web. In addition, removal of toxic metals from the environment by plants or plant products has potential as an effective means of remediating heavy metal and radionuclide waste.

耐金属植物通过排除或减少吸收来发挥作用。 有毒离子，或在细胞壁中积累金属，或络合 以某种方式降低其毒性。无毒曼陀罗 是一种能合成多肽的耐镉植物。 在电池内部隔离镉并螯合重金属 细胞壁上的外部离子。金属结合的性质 细胞壁上的位置尚不清楚，然而，有一些 源于细胞壁蛋白质、脂类和 能够配合金属离子的碳水化合物。我们的目标是利用 直接探测金属结合的X射线吸收光谱 无毒拟青霉细胞壁上的位点，特别是Ba2+，Cu2+和Cd2+金属 结合部位。了解植物细胞壁之间的相互作用 重金属离子很重要，因为金属在 农业上重要的植物是重振旗鼓的主要手段 金属被引入食物链。此外，删除 植物或植物产品从环境中排出的有毒金属 作为修复重金属和环境污染的有效手段 放射性核素废物。