ANIMAL NEUROIMAGING AND CELLULAR PHYSIOLOGY

动物神经成像和细胞生理学

• 批准号: 6587591
• 负责人: Robert D Frisina
• 金额: $ 23.07万
• 依托单位: ROCHESTER INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6587591
• 关键词: aging; animal old age; auditory nuclei; auditory stimulus; calbindin; calcium; calcium indicator; calretinin; cochlea; ear hair cell; electrophysiology; ethology; gene expression; immunocytochemistry; inferior colliculus; juvenile animal; laboratory mouse; neural information processing; neuroanatomy; neurons; neurophysiology; noise; presbycusis; tissue /cell culture; western blottings

Hearing loss in the elderly, presbycusis, is the number one communicative disorder of the elderly. Classically, loss of sensory cells (hair cells) in the basal (high pitch) portion of the cochlear (inner ear of the peripheral auditory system) has contributed to presbycusis. Other more recent evidence points to age-related changes in the brain itself (central auditory system) as a cause of presbycusis. These two factors result in the two main perceptual difficulties: a high-pitch loss in sensitivity and a difficulty of understanding speech in background noise. During the previous funding period, significant age-related changes were observed in animals with age-related auditory temporal pathways, electron microscopy of midbrain synapses, cochlear inner and outer hair cell degeneration, and others. In the upcoming grant period, techniques of cellular neuroimaging will be applied in slice preparations of young and old animals to determine calcium regulatory changes with age in brain regions we previously demonstrated had age-related temporal processing deficits, as well as determining if manipulation of intracellular calcium concentration levels and stores will affect the processing of auditory temporal information. Close ties will exist with the Animal Behavior Project (acoustic startle response- Project 2), the Single-unit Physiology Project (midbrain, cochlear nucleus- Project 3), and the Human and Animal Evoked Potential Project (animal Wave I and IV- Project 5). These neurophysiological and behavioral projects will evaluate any age-related improvements in temporal processing in old CBA mice that may occur due to loading the inferior colliculus (IC) of young, adult mice with increased calcium regulators in the present project. In addition, HRP immunohistochemistry will continue to be performed in conjunction with the single-unit neurophysiological mapping experiments of Project 3. Specifically, inputs to the ventrolateral division of the central nucleus of IC will be compared to the decline inputs to dorsomedial IC that we have already discovered with age. Descending inputs to the central cochlear nucleus from higher centers of the brain will be examined to see if any age-related changes in connectivity occur. The results of these studies will be utilized to prepared for medical/surgical/technological interventions to increase the quality of life in our elderly population, especially in regard to sensory perception and brain functioning.

老年人的听力损失，老年性耳聋，是头号沟通 老年人的疾病。感觉细胞（毛细胞）的丧失 在耳蜗的基底（高音）部分（内耳的 周围听觉系统）导致了老年性耳聋。其他更 最近的证据表明，大脑本身（中枢神经系统）发生了与年龄相关的变化。 听觉系统）作为老年性耳聋的原因。这两个因素导致 两个主要的感知困难：高音调的灵敏度损失， 在背景噪音中理解语言的困难。期间 在上一个资助期间， 具有年龄相关听觉颞叶通路的动物，电子显微镜 中脑突触，耳蜗内外毛细胞变性， 他人在即将到来的资助期内，细胞神经成像技术 将应用于幼龄和老龄动物的切片制剂中， 确定钙调节的变化与年龄的大脑区域，我们 先前证明有年龄相关的时间处理缺陷， 以及确定是否操纵细胞内钙浓度 水平和存储会影响听觉时间的加工 信息.将与动物行为项目保持密切联系 （声音惊吓反应-项目2），单单位生理学项目 （中脑，耳蜗核-项目3），以及人类和动物的诱发 潜力项目（动物第一和第四波-项目5）。这些 神经生理学和行为学项目将评估任何与年龄有关的 老年CBA小鼠时间处理的改善，可能是由于 给年轻的成年小鼠的下丘（IC）加载增加的 钙调节剂在本项目中。此外，HRP 免疫组织化学将继续与 项目3的单单位神经生理学映射实验。 具体来说，中央核腹外侧区的输入 将IC的下降输入到背内侧IC，我们 已经随着年龄的增长而发现。降序输入到中央 将检查来自大脑高级中心的耳蜗核， 如果连接发生任何与年龄相关的变化。的结果予以 研究将用于为医疗/手术/技术准备 提高老年人口生活质量的干预措施， 特别是在感官知觉和大脑功能方面。