ANIMAL NEUROIMAGING AND CELLULAR PHYSIOLOGY

动物神经成像和细胞生理学

• 批准号: 6587591
• 负责人: Robert D Frisina
• 金额: $ 23.07万
• 依托单位: ROCHESTER INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6587591
• 关键词: aging; animal old age; auditory nuclei; auditory stimulus; calbindin; calcium; calcium indicator; calretinin; cochlea; ear hair cell; electrophysiology; ethology; gene expression; immunocytochemistry; inferior colliculus; juvenile animal; laboratory mouse; neural information processing; neuroanatomy; neurons; neurophysiology; noise; presbycusis; tissue /cell culture; western blottings

Hearing loss in the elderly, presbycusis, is the number one communicative disorder of the elderly. Classically, loss of sensory cells (hair cells) in the basal (high pitch) portion of the cochlear (inner ear of the peripheral auditory system) has contributed to presbycusis. Other more recent evidence points to age-related changes in the brain itself (central auditory system) as a cause of presbycusis. These two factors result in the two main perceptual difficulties: a high-pitch loss in sensitivity and a difficulty of understanding speech in background noise. During the previous funding period, significant age-related changes were observed in animals with age-related auditory temporal pathways, electron microscopy of midbrain synapses, cochlear inner and outer hair cell degeneration, and others. In the upcoming grant period, techniques of cellular neuroimaging will be applied in slice preparations of young and old animals to determine calcium regulatory changes with age in brain regions we previously demonstrated had age-related temporal processing deficits, as well as determining if manipulation of intracellular calcium concentration levels and stores will affect the processing of auditory temporal information. Close ties will exist with the Animal Behavior Project (acoustic startle response- Project 2), the Single-unit Physiology Project (midbrain, cochlear nucleus- Project 3), and the Human and Animal Evoked Potential Project (animal Wave I and IV- Project 5). These neurophysiological and behavioral projects will evaluate any age-related improvements in temporal processing in old CBA mice that may occur due to loading the inferior colliculus (IC) of young, adult mice with increased calcium regulators in the present project. In addition, HRP immunohistochemistry will continue to be performed in conjunction with the single-unit neurophysiological mapping experiments of Project 3. Specifically, inputs to the ventrolateral division of the central nucleus of IC will be compared to the decline inputs to dorsomedial IC that we have already discovered with age. Descending inputs to the central cochlear nucleus from higher centers of the brain will be examined to see if any age-related changes in connectivity occur. The results of these studies will be utilized to prepared for medical/surgical/technological interventions to increase the quality of life in our elderly population, especially in regard to sensory perception and brain functioning.

老年人的听力损失，长老会，是第一名 老年人。经典，感觉细胞的丧失（毛细胞） 在人工耳蜗的基础（高螺距）部分（ 外围听觉系统）已促成长老会。其他 最近的证据表明大脑本身与年龄相关的变化（中心 听觉系统）是老年人的原因。这两个因素导致 这两个主要的感知困难：灵敏度和 在背景噪音中理解语音的困难。在 以前的资金期，观察到与年龄有关的重大变化 具有与年龄相关的听觉时间通路，电子显微镜的动物 中脑突触，人工耳蜗内部和外毛细胞变性，以及 其他的。在即将到来的授予期内，细胞神经影像学技术 将应用于年轻动物的切片制剂 确定钙调节性变化随着大脑区域的年龄而变化 以前证明的与年龄相关的时间处理缺陷 以及确定是否操纵细胞内钙浓度 级别和商店将影响听觉时间的处理 信息。动物行为项目将存在紧密的联系 （声学惊吓响应 - 项目2），单单体生理项目 （中脑，耳蜗核 - 项目3），人类和动物唤起了 潜在项目（动物波和IV-项目5）。这些 神经生理和行为项目将评估任何与年龄有关的 旧CBA小鼠的时间处理改善 加载年轻小鼠的下丘（IC） 钙调节剂在本项目中。另外，HRP 免疫组织化学将继续与 项目3的单个单元神经生理映射实验。 具体而言，中央核的腹外侧分裂的输入 IC的下降将与我们的背部IC的下降输入进行比较。 随着年龄的增长已经发现。下达中央的输入 将检查来自大脑较高中心的耳蜗核，以查看 如果发生任何与年龄相关的连通性变化。这些结果 研究将用于准备医学/外科/技术 提高老年人口生活质量的干预措施， 特别是关于感官感知和大脑功能。