The design and manufacture of 3D peptide gels for tissue engineering applications

用于组织工程应用的 3D 肽凝胶的设计和制造

基本信息

  • 批准号:
    2111185
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Studentship
  • 财政年份:
    2018
  • 资助国家:
    英国
  • 起止时间:
    2018 至 无数据
  • 项目状态:
    已结题

项目摘要

The cornea is a transparent window located at the front of the eye and has several roles imperative to the main functions of the eye. These include controlling the amount of light reaching the back of the eye, focusing light onto the retina and establishing and maintaining the eye shape. It is composed of three main layers: a stratified epithelium, the stroma, and a single layered endothelium. A damaged cornea can result in blindness, and the most common treatment for this is donor cornea replacement. Some pathologies where a replacement cornea may be required include chemical burns, Steven-Johnson syndrome and autoimmune diseases such as mucus membrane pemphigoid. This procedure is widely used throughout the world, but is not without its limitations. Amongst these are the limited availability of the donor corneas, issues with tissue rejection and infection and finally, the expense of the procedure. An artificial cornea would be able to combat these limitations, as they could be made of a biocompatible synthetic material reducing tissue rejection, they could be manufactured in large volumes and could eliminate expenses such as donor cornea screening.A recent study at the University of Liverpool developed a poly-E-lysine (pEK) based hydrogel material that was seen to be effective for use in anti-microbial contact lenses. This polymer is based on pEK cross-linked with bis-carboxy fatty acids, the most suitable found to be suberic acid. An optimal monomer composition was established and found to be non-cytotoxic to HCE-T cells and did not inhibit the re-epithelialisation of a cell monolayer. A hydrogel is a water-swollen network of polymer crosslinks, which maintains physical and mechanical properties that make it a desirable material for biomaterial and tissue engineering applications. Their high water content allows them to be introduced into a biological environment without affecting the viability, and also makes them suitable for cell attachment. The mechanical properties of poly-E-lysine specifically can be tailored by altering both the polymer and cross-linking density and the molecular length of the cross linker. For instance, increasing the polymer density directly increases the ultimate tensile strength of the final hydrogel.The aim of this project is to produce a 3D macro-porous form of this pEK based hydrogel previously tested as a thin film. The design and manufacture of this 3D porous gel will be trialled via a number of routes. These include the controlled 3D printing of porous constructs, the crosslinking of hydrogel fragments and forms of gel casting. This will involve exploring several different printing technologies and determining what is the most suitable for this material. The hydrogel chemistry can further be altered to suit these different technologies and to optimise cell attachment and response. Upon establishing a suitable manufacturing process, the properties of the material could be altered to suit applications within the eye, with the main focus being transparency. Finally, several trials will be performed on the material with this modified architecture to assess its suitability for cell incorporation and attachment. This material could inevitably be used for an artificial cornea, or an in vitro 3D tissue model of the cornea when cells are incorporated.This project will take place between the Institute of Ageing and Chronic Disease and the School of Engineering and will be supervised by Professor Rachel Williams and Dr Kate Black.
角膜是位于眼睛前部的透明窗口,对眼睛的主要功能有几个重要的作用。这包括控制到达眼睛后部的光线量,将光线聚焦到视网膜上,以及建立和保持眼睛的形状。它主要由三层组成:层状上皮、间质和单层内皮。角膜受损会导致失明,最常见的治疗方法是角膜移植。一些可能需要更换角膜的病理包括化学烧伤、史蒂文-约翰逊综合征和自身免疫性疾病,如粘液膜类天疱疮。这一程序在世界各地广泛使用,但并非没有其局限性。其中包括供体角膜的有限供应,组织排斥和感染的问题,最后是手术的费用。人造角膜将能够克服这些限制,因为它们可以由生物相容性的合成材料制成,减少组织排斥,它们可以大批量生产,并且可以消除诸如供体角膜筛选之类的费用。利物浦大学最近的一项研究开发了一种基于聚e -赖氨酸(pEK)的水凝胶材料,被认为可以有效地用于抗菌隐形眼镜。该聚合物是基于pEK交联双羧基脂肪酸,最合适的发现是亚酰基酸。建立了最佳单体组成,发现对HCE-T细胞无细胞毒性,并且不抑制细胞单层的再上皮化。水凝胶是一种水膨胀的聚合物交联网络,它保持了物理和机械性能,使其成为生物材料和组织工程应用的理想材料。它们的高含水量允许它们被引入生物环境而不影响生存能力,也使它们适合细胞附着。聚e -赖氨酸的机械性能可以通过改变聚合物和交联密度以及交联剂的分子长度来定制。例如,增加聚合物密度直接增加最终水凝胶的极限拉伸强度。该项目的目的是生产一种3D大孔形式的基于pEK的水凝胶,这种水凝胶之前被测试为薄膜。这种3D多孔凝胶的设计和制造将通过多种途径进行试验。这些包括多孔结构的受控3D打印,水凝胶碎片的交联和凝胶铸造的形式。这将涉及探索几种不同的印刷技术,并确定哪种技术最适合这种材料。水凝胶的化学性质可以进一步改变,以适应这些不同的技术,并优化细胞的附着和反应。在建立合适的制造工艺后,材料的特性可以改变以适应眼睛内的应用,主要焦点是透明度。最后,将对具有这种修改结构的材料进行几次试验,以评估其对细胞结合和附着的适用性。这种材料不可避免地会被用于人造角膜,或者当细胞被纳入角膜的体外3D组织模型。该项目将由老龄化和慢性疾病研究所和工程学院共同进行,并将由Rachel Williams教授和Kate Black博士监督。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

其他文献

吉治仁志 他: "トランスジェニックマウスによるTIMP-1の線維化促進機序"最新医学. 55. 1781-1787 (2000)
Hitoshi Yoshiji 等:“转基因小鼠中 TIMP-1 的促纤维化机制”现代医学 55. 1781-1787 (2000)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
LiDAR Implementations for Autonomous Vehicle Applications
  • DOI:
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
生命分子工学・海洋生命工学研究室
生物分子工程/海洋生物技术实验室
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
吉治仁志 他: "イラスト医学&サイエンスシリーズ血管の分子医学"羊土社(渋谷正史編). 125 (2000)
Hitoshi Yoshiji 等人:“血管医学与科学系列分子医学图解”Yodosha(涉谷正志编辑)125(2000)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Effect of manidipine hydrochloride,a calcium antagonist,on isoproterenol-induced left ventricular hypertrophy: "Yoshiyama,M.,Takeuchi,K.,Kim,S.,Hanatani,A.,Omura,T.,Toda,I.,Akioka,K.,Teragaki,M.,Iwao,H.and Yoshikawa,J." Jpn Circ J. 62(1). 47-52 (1998)
钙拮抗剂盐酸马尼地平对异丙肾上腺素引起的左心室肥厚的影响:“Yoshiyama,M.,Takeuchi,K.,Kim,S.,Hanatani,A.,Omura,T.,Toda,I.,Akioka,
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:

的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('', 18)}}的其他基金

An implantable biosensor microsystem for real-time measurement of circulating biomarkers
用于实时测量循环生物标志物的植入式生物传感器微系统
  • 批准号:
    2901954
  • 财政年份:
    2028
  • 资助金额:
    --
  • 项目类别:
    Studentship
Exploiting the polysaccharide breakdown capacity of the human gut microbiome to develop environmentally sustainable dishwashing solutions
利用人类肠道微生物群的多糖分解能力来开发环境可持续的洗碗解决方案
  • 批准号:
    2896097
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
A Robot that Swims Through Granular Materials
可以在颗粒材料中游动的机器人
  • 批准号:
    2780268
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Likelihood and impact of severe space weather events on the resilience of nuclear power and safeguards monitoring.
严重空间天气事件对核电和保障监督的恢复力的可能性和影响。
  • 批准号:
    2908918
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Proton, alpha and gamma irradiation assisted stress corrosion cracking: understanding the fuel-stainless steel interface
质子、α 和 γ 辐照辅助应力腐蚀开裂:了解燃料-不锈钢界面
  • 批准号:
    2908693
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Field Assisted Sintering of Nuclear Fuel Simulants
核燃料模拟物的现场辅助烧结
  • 批准号:
    2908917
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Assessment of new fatigue capable titanium alloys for aerospace applications
评估用于航空航天应用的新型抗疲劳钛合金
  • 批准号:
    2879438
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Developing a 3D printed skin model using a Dextran - Collagen hydrogel to analyse the cellular and epigenetic effects of interleukin-17 inhibitors in
使用右旋糖酐-胶原蛋白水凝胶开发 3D 打印皮肤模型,以分析白细胞介素 17 抑制剂的细胞和表观遗传效应
  • 批准号:
    2890513
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
CDT year 1 so TBC in Oct 2024
CDT 第 1 年,预计 2024 年 10 月
  • 批准号:
    2879865
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Understanding the interplay between the gut microbiome, behavior and urbanisation in wild birds
了解野生鸟类肠道微生物组、行为和城市化之间的相互作用
  • 批准号:
    2876993
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship

相似海外基金

Pre-clinical validation of 3D-printed nerve conduits for pediatric peripheral nerve repair
3D 打印神经导管用于儿科周围神经修复的临床前验证
  • 批准号:
    10672031
  • 财政年份:
    2023
  • 资助金额:
    --
  • 项目类别:
3D Printed Microfluidic Artificial Lung for Veteran Rehabilitation
用于退伍军人康复的 3D 打印微流控人工肺
  • 批准号:
    10629531
  • 财政年份:
    2023
  • 资助金额:
    --
  • 项目类别:
Development of a 3D neurovascular unit for in vitro modeling of subarachnoid hemorrhage and screening therapies
开发用于蛛网膜下腔出血体外建模和筛选治疗的 3D 神经血管单元
  • 批准号:
    10722387
  • 财政年份:
    2023
  • 资助金额:
    --
  • 项目类别:
3D microprinting-enabled microinjection needle arrays for enhanced therapeutics delivery into the brain
支持 3D 微打印的显微注射针阵列可增强向大脑的治疗输送
  • 批准号:
    10761073
  • 财政年份:
    2023
  • 资助金额:
    --
  • 项目类别:
3D Printed Configurable and Themoresponsive Intracortical Electrode Array Platform
3D 打印可配置和热响应皮质内电极阵列平台
  • 批准号:
    10883867
  • 财政年份:
    2023
  • 资助金额:
    --
  • 项目类别:
ELIMINATION OF AIRBORNE VOLATILE COMPOUNDS THROUGH INCORPORATION OF ADVANCED 3D NANOSTRUCTURED CATALYTIC COATINGS IN ADSORPTION/DECOMPOSITION AIR PURIFICATION SYSTEMS
通过在吸附/分解空气净化系统中采用先进的 3D 纳米结构催化涂层消除空气中的挥发性化合物
  • 批准号:
    10556402
  • 财政年份:
    2022
  • 资助金额:
    --
  • 项目类别:
3D Bioprinted Collagen Vascular Conduits For Use In Patients With Congenital Heart Defects
3D 生物打印胶原血管导管用于先天性心脏病患者
  • 批准号:
    10763791
  • 财政年份:
    2022
  • 资助金额:
    --
  • 项目类别:
Commercialization readiness for a 3D image guidance system to support interventional procedures in the spine and pelvis
支持脊柱和骨盆介入手术的 3D 图像引导系统已做好商业化准备
  • 批准号:
    10709021
  • 财政年份:
    2022
  • 资助金额:
    --
  • 项目类别:
3D Bioprinting of Strong Living Scaffolds
坚固生命支架的 3D 生物打印
  • 批准号:
    10682568
  • 财政年份:
    2022
  • 资助金额:
    --
  • 项目类别:
Patient Specific 3D Printed Diabetic Insoles to Reduce Plantar Pressure
患者专用 3D 打印糖尿病鞋垫可减少足底压力
  • 批准号:
    10609500
  • 财政年份:
    2022
  • 资助金额:
    --
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了