CORE--SCIENTIFIC

核心--科学

• 批准号: 6668220
• 负责人: Patricia Ann D'Amore
• 金额: $ 9.16万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6668220
• 关键词: angiogenesis; bioimaging /biomedical imaging; biomedical facility; chick embryo; confocal scanning microscopy; histology; image processing; immunocytochemistry; photography; tissue /cell culture; tissue /cell preparation; vascular endothelium

The goal of the Scientific Core is to supportand facilitate the research programs of the investigators in the program project. The overall Scientific Core consists of a Photography Sub-Core, an Angiogenesis Assay Sub-Core,andan imaging Sub-Core. (i) The Photography Core provides high quality, in-house conventional and digital services, including figures for publication, and imagesfor slide and Power Point presentations. This Core is staffed by one full-time photographer with over3 years experience in the department and a parttime assistant. (ii) The Angiogenesis Assay Core will provide microvascular endothelial cells and expertise in carrying out angiogenesis assays, including chick aortic rings to measure angiogenesis in vitro, and the chick chorioallantoic membrane assay to test angiogenesis promoters and inhibitors in vivo. This Core is staffed by two senior technicians, each with over 15 years experience, one in large and small vessel endothelial cell preparation and biology, and the other in the developing chick embryo system. (iii)The imaging Core is designed to transform the original histology core into a modem high-resolution operation. This Core will provide a centralized facility for taser-confocal microscopy, real-time imaging, and three-dimensional reconstruction of living ceils, whole embryos, and histological sections of tumors and developing vascular tissue. The Imaging Core will be staffed by an experienced confocal microscopist who will operate the shared laser-scanning confocal microscope, instruct and assist Project members in its use, and manage access to the facility. This new facility is critical to a number of the specific aims of the Program Project.

科学核心的目的是支持和促进计划项目中研究人员的研究计划。总体科学核心由摄影子核，一种血管生成测定子核，Andan Imaging子核组成。 （i）摄影核心提供高质量的内部传统和数字服务，包括出版物的数字以及幻灯片和电源点演示的图像。该核心由一位全职摄影师组成，拥有超过3年的部门经验和一名兼职助理。 （ii）血管生成测定的核心将在进行血管生成测定方面提供微血管内皮细胞和专业知识，包括在体外测量血管生成的雏鸡主动脉环，以及在体内测试血管生成的鸡绒毛膜膜膜分析。该核心由两名高级技术人员组成，每个技术人员都有15年以上的经验，一个在大型和小型船只内皮细胞的制备和生物学中，另一个在发育中的小鸡胚胎系统中。 （iii）成像芯旨在改变原始的组织学 核心进入现代高分辨率操作。该核心将为TASER-CONFOCAL显微镜，实时成像以及生物天花板，整个胚胎以及肿瘤的组织学切片和发展血管组织的三维重建提供集中式设施。成像核心将由经验丰富的共聚焦显微镜组成，他们将操作共享的激光扫描共聚焦显微镜，指导和协助项目成员使用并管理对设施的访问。 这个新设施对于计划项目的许多特定目标至关重要。