UCLA Imaging Resource for Mouse Cancer Models

描述（申请人提供）：UCLA拥有成熟的小动物成像 基于数字全身放射自显影（DWBA）和微正电子的程序 发射断层扫描（microPET）。我们在这项研究上投入了大量资金 小动物癌症模型的研究。记者 基因与DWBA和microPET的结合为我们提供了 研究癌症生物学，细胞运输和基因临床前模型 疗法正是通过这些研究，我们更好地了解了 我们现有技术的局限性，因此建议收购 微型计算机断层扫描（microCAT）和光学电荷耦合器件 (CCD)成像系统。我们还将获得一台急需的电脑 服务器和数据归档系统，用于生成大量数据 通过这项工作。我们将允许六名经验丰富的癌症研究人员 利用资源，然后通过增加三名调查员， 年 MicroCAT将使我们能够对小鼠癌症的潜在解剖结构进行成像 模型这将是至关重要的，以帮助我们了解的位置（S） 而不需要牺牲动物。此外 通过这项工作中提出的直接研究，我们将注册microPET 和microCAT信息，为我们的研究人员提供最大的信息 关于功能和解剖学的。光学CCD系统也将是关键， 帮助我们加速癌症相关研究。萤火虫的用途 荧光素酶（FLUC）报告基因将使我们能够更快地研究小 没有放射性探针的动物模型。该系统具有以下能力： 从一个活着的小动物体内成像低水平的光。我们将使用 报告基因系统，将FLUC和PET报告基因偶联，以便具有 在光学CCD系统或microPET中成像的灵活性。这 将有助于加快发展各种模式， 报告基因技术所有模态的数据定量将 在整个SAIRP中始终强调。 我们还将制定一个强有力的培训计划，帮助调查人员 和他们的学生成为独立和自信地使用现有的 资源整个资源的使用将由基于互联网的 调度软件和监督委员会我们相信，新的 微CAT和光学CCD技术的资源将有助于为UCLA提供 研究人员使用最先进的工具对小鼠癌症进行非侵入性成像 模型

DESCRIPTION (provided by applicant): UCLA has a mature small animal imaging program based on digital whole body autoradiography (DWBA) and micro positron emission tomography (microPET). We have significant investments in the study of small animal cancer models using PET reporter gene technology. Reporter genes in combination with DWBA and microPET have provided us the ability to study cancer biology, cell trafficking, and pre-clinical models for gene therapy. It is through these studies that we have better understood the limitations of our current technologies and therefore propose the acquisition of micro computed tomography (microCAT) and optical charge coupled device (CCD) imaging systems. We will also acquire a critically needed computer server and data archiving system for the large amounts of data generated through this work. We will initially allow six seasoned cancer investigators to use the resource and then grow by adding up to three investigators per year. MicroCAT will allow us to image the underlying anatomy in our mouse cancer models. This will be critical in helping us to understand the location(s) of various cellular events without the need to sacrifice the animals. Furthermore through direct research proposed in this work we will register the microPET and microCAT information to provide our researchers with maximal information on function and anatomy. The optical CCD system will also be critical in helping to accelerate our cancer related research. The use of the firefly luciferase (FLUC) reporter gene will allow us to more rapidly study small animal models without radioactive probes. This system has the capability of imaging low levels of light from within a living small animal. We will use reporter systems that couple FLUC and PET reporter genes in order to have the flexibility to image in either an optical CCD system or the microPET. This will help to accelerate the development of various models that are dependent on reporter gene technology. Quantitation of data from all modalities will always be stressed throughout the SAIRP. We will also develop a strong training program that will help investigators and their students to become independent and confident in using the available resources. Use of the entire resource will be coordinated by intemet based scheduling software and an oversight committee. We are confident that the new resources with microCAT and optical CCD technologies will help to provide UCLA investigators with state-of-art tools for non-invasively imaging mouse cancer models.