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Characterisation of the N-end rule pathway and its substrates as a modulator of the plant immune response

N 端规则途径及其底物作为植物免疫反应调节剂的表征

基本信息

批准号：
2117721
负责人：
金额：
--
依托单位：
University of Nottingham
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2117721
关键词：
Characterisation end rule pathway its

项目摘要

We previously provided the first evidence for functions for the N-end rule pathway of ubiquitin-mediated targeted proteolysis in plants (Gibbs et al Nature 2011; Gibbs et al Molecular Cell 2014; Abbas et al Current Biology 2015, Vicente et al Current Biology 2017). This pathway regulates the stability of regulatory proteins in eukaryotic cells, and is a key regulator of growth and development. The pathway controls protein degradation dependent on the amino (N)-terminal (Nt-) residue of proteins. We have shown that one branch of this pathway, controlled by a Glutamine Amido-hydrolase (NTAQ), controls the plant immune response. This is the first time that a function for this branch of the pathway has been discovered in plants. This project will focus on discovering protein substrates of NTAQ and understanding their contribution to plant defence responses. The student will learn molecular genetics of Arabidopsis thaliana, as well as developing theoretical and practical understanding of plant pathology. Molecular biology, proteomics and Western blotting will be used to validate mutants, analyse genetic stress markers and probe for new protein substrates. This study will reveal new functions for the N-end rule pathway, determining the relationship between newly discovered NTAQ substrates and the known plant immune network.

我们之前提供了植物中泛素介导的靶向蛋白水解的N端规则途径功能的第一个证据（Gibbs et al Nature 2011; Gibbs et al Molecular Cell 2014; Abbas et al Current Biology 2015，Vicente et al Current Biology 2017）。该途径调节真核细胞中调节蛋白的稳定性，并且是生长和发育的关键调节因子。该途径依赖于蛋白质的氨基（N）-末端（Nt-）残基来控制蛋白质降解。我们已经表明，该途径的一个分支，由谷氨酰胺水解酶（NTAQ）控制，控制植物免疫反应。这是首次在植物中发现该途径这一分支的功能。该项目将集中于发现NTAQ的蛋白质底物，并了解它们对植物防御反应的贡献。学生将学习拟南芥的分子遗传学，以及对植物病理学的理论和实践了解。分子生物学、蛋白质组学和蛋白质印迹法将用于验证突变体、分析遗传应激标记和探测新的蛋白质底物。这项研究将揭示N-末端规则途径的新功能，确定新发现的NTAQ底物与已知植物免疫网络之间的关系。