All-in-One Biochip for Infectious Diseases Screening

描述（由申请人提供）：在美国，每三秒钟就有一个人需要捐献血液，这相当于每天使用大约40,000品脱的血液。目前用于血液筛查的实验室检测非常耗时，而且必须对所有传染病进行“连续”检测。现场快速识别患有血液传播疾病的潜在献血者，将有助于消除不必要的抽血、处理、储存和运输成本，并有助于防止进一步感染。该项目将开发一种多功能传染病芯片（IDChip）技术，该技术可以快速、灵敏、“同时”检测和识别多种（大约10种）血液传播疾病。这种一体化IDChip包含所有必需的试剂，因此从手指棒加载非常小的体积是唯一需要手动参与。这种一次性IDChip利用微加工和集成的微流体技术，在光盘包装中提供超灵敏的生物化学自动化。IDChip系统紧凑、便携，将有助于确保国家的血液供应不受传染病的影响。第一阶段的工作将集中在IDChip平台的设计和制造，压力驱动微流体的构建，系统的集成，生物检测（HBsAg，抗hcv，抗hav， HIV-1）的性能，以及系统性能的评估。

DESCRIPTION (provided by applicant): Every three seconds someone in the United States requires donated blood, which correlates to approximately 40,000 pints of blood used each day. Current laboratory tests for blood screening are time consuming and must be performed "consecutively" for all infectious diseases. Rapid onsite identification of potential donors with blood borne diseases, will help to eliminate needless blood drawn, handling, storage, transportation costs, and will help to prevent further infections. This project will develop an all-in-one infectious diseases chip (IDChip) technology that can rapidly, sensitively, and "simultaneously" detect and identify multiple (approximately 10) blood transmissible diseases. This all-in-one IDChip contains all required reagents, so loading a very small volume from a finger-stick is the only manual involvement required. This disposable IDChip utilizes microfabrication and integrated microfluidic technologies to provide ultra-sensitive biochemistry automation in a disc package. The IDChip system is compact, portable, and will help to ensure that the nation's blood supply is free from infectious diseases. Phase I work will focus on design and fabrication of the IDChip platform, construction of pressure-driven microfluidics, integration of the system, performance of bioassays (HBsAg, anti-HCV, anti-HAV, HIV-1), and evaluation of the system's performance.