All-in-One Biochip for Infectious Disease Screening

描述（由申请人提供）：在美国，每三秒钟就有一个人需要捐献血液，这相当于每天使用大约40,000品脱的血液。目前用于血液筛查的实验室检测非常耗时，而且必须对所有传染病进行“连续”检测。现场快速识别患有血液传播疾病的潜在献血者将有助于消除不必要的抽血、处理、储存和运输成本，并有助于预防进一步感染。该项目将开发一种能够快速、灵敏、“同时”检测和识别多种（6-10）血液传播疾病的一体化传染病芯片（IDChip）技术。这种一体化IDChip包含所有必需的试剂，因此从手指棒加载非常小的体积是唯一需要手动参与。这种一次性IDChip利用微加工和集成的微流体技术，在光盘包装中提供超灵敏的生物化学自动化。IDChip系统紧凑、便携，将有助于确保国家的血液供应不受传染病的影响。

DESCRIPTION (provided by applicant): Every three seconds someone in the United States requires donated blood, which correlates to approximately 40,000 pints of blood used each day. Current laboratory tests for blood screening are time consuming and must be performed "consecutively" for all infectious diseases. Rapid on-site identification of potential donors with blood borne diseases will help to eliminate needless blood draws, handling, storage, and transportation costs, and will help to prevent further infections. This project will develop an all-in-one infectious diseases chip (IDChip) technology that can rapidly, sensitively, and "simultaneously" detect and identify multiple (6-10) blood transmissible diseases. This all-in-one IDChip contains all required reagents, so loading a very small volume from a finger-stick is the only manual involvement required. This disposable IDChip utilizes microfabrication and integrated microfluidic technologies to provide ultrasensitive biochemistry automation in a disc package. The IDChip system is compact, portable, and will help to ensure that the nation's blood supply is free from infectious diseases. During the Phase I project, we have designed and fabricated a microfluidic based IDChip, constructed a laboratory system, and characterized the system with three targets: Hepatitis B Surface antigen (HBsAg), Hepatitis B Core Antibody (HBcAb), and Hepatitis C Virus Antibody (HCVAb), performed control experiments, and demonstrated the assay capabilities. The proposed IDChip system not only automated a rapid test (< 25 minutes), but also offers excellent sensitivity.