ENHANCEMENT OF FLUORESCENCE FOR CLINICAL DIAGNOSIS

光学探测器技术已经发展到了这样一个地步 单光子计数能力。从理论上讲， 光学诊断要达到足够的灵敏度才能检测到单个 分子。不幸的是，生物的高度分散性 样品和光学的高度衍射性(或反射性) 元素会产生非常严重的噪音和背景问题，特别是对于 低信号强度区域。为了缓解这个问题，物理上 光学公司(POC)建议继续开发一种特殊的 全息降噪涂层技术，类似于蛾眼 结构，这显著提高了光子检测灵敏度。 在此项目的第一阶段，POC成功展示了 这些涂层的可行性和通用性，并表明它们可以 应用于光学检测系统的几乎任何内部部件， 将灵敏度提高高达10倍。PoC的新奇特性 基于蛾眼的技术包括：L)薄全息薄膜 作为高效的捕光器；2)薄膜在以下情况下具有高透明度 作为减反射膜；3)薄膜明显增大 由于表面起伏而产生的表面积；4)随机化的蛾眼 结构充当漫射器，将入射光均匀化为 受控制的空间模式。 建议的商业应用：POC的低成本蛾眼涂料 具有大规模生产潜力的技术提供了显著改进 灵敏度，增强了对微弱光信号的检测。取决于 表面浮雕结构的设计，以蛾眼箔为主可有 各种新奇的特性。特别是，蛾眼箔将是 在流式细胞术中实施(以减少杂散光)，固相 免疫分析(检测表面强化)、内窥镜检查(产生均匀的 照明)、测光和光谱分析(以减少光散射)。

Optical detector technology has progressed to the point where it has single photon counting capability. It is theoretically possible for optical diagnosis to achieve enough sensitivity to detect a single molecule. Unfortunately, the highly scattering nature of biological samples and the highly diffractive (or reflective) nature of optical elements create very serious noise and background problems, especially for the low signal intensity regime. To alleviate this problem, Physical Optics Corporation (POC) proposes to continue the development of a special holographic noise suppressing coating technology, analogous to moth-eye structures, which significantly improves photon detection sensitivity. During Phase I of this project, POC successfully demonstrated the feasibility and versatility of these coatings, and showed that they can be applied to almost any internal part of an optical detection system, improving sensitivity by up to a factor of 10. Novelty features of POC's moth-eye based technology include the following: l) Thin holographic films act as highly effective light-traps; 2) Films have high transparency when acting as antireflection coatings; 3) Films have significantly enlarged surface area due to their surface relief; and 4) Randomized moth-eye structures act as a diffuser that will homogenize incoming light into a controlled spatial pattern. PROPOSED COMMERCIAL APPLICATION: POC's low cost moth-eye coating technology, with mass-production potential, offers significantly improved sensitivity, enhancing the detection of weak optical signals. Depending on the design of the surface relief structure, moth-eye based foils can have various novel properties. In particular, the moth-eye foil will be implemented in flow cytometry (to reduce stray light), solid phase immunoassay (to sense surface enhancements), endoscopy (to produce uniform illumination), photometry and spectrometry (to reduce light scattering).