Image-coded Microsphere for Multiplexed Bioassay

描述（由申请人提供）：由于目前基因组学和蛋白质组学的研究产生了更多的多路数据，因此需要能够在极少量样品中快速筛选大量核酸和蛋白质的技术。微阵列和DNA芯片是在平面平台上进行的，将小体积的液体样品分布在相对较大的芯片表面上，往往会遇到样品数量不足或分布不均匀的问题。MSI建议开发一种微图像编码微球技术，用于使用前所未有的微体积样品进行多种生物分析。所提出的技术是基于光学条形码粒子，通过将纳米或微图像插入乳胶珠壳而形成。这项技术的新颖之处在于，图像编码的珠子可以识别表面上发生的反应。每个微珠执行一次测试，因此作为一个单一的分析分析仪。通过添加微粒子的混合物，可以同时检测数千种分析物，方便、快速、廉价。

DESCRIPTION (provided by applicant): As current research in genomics and proteomics produce more multiplexed data, there is a need for technologies that can rapidly screen a large number of nucleic acids and proteins in a very small volume of sample. Microarray and DNA chips are performed on a planar platform, distributing a small volume of liquid sample over a relatively large chip surface often encounter the problems of an insufficient sample amount or non-uniform distribution. MSI proposes to develop a micro-image-coded microsphere technology for multiplexed thousands of bioassays using an unprecedented level of micro-volume samples. The proposed technology is based on optically bar-coded particles, formed by inserting nano or micro images into a latex bead shell. The novelty of this technology is that image-coded beads identify which reaction is taking place on the surface. Each microbead performs one test, thus acting as a single analyte analyzer. By adding a mixture of micro particles, several thousands analytes can be tested simultaneously, easily, rapidly, and inexpensively. Phase I work will focus on image-coded particle synthesis, micro image recording, optical decoding, and DNA hybridization assays. In Phase II, the technology will be applied to cellular analysis, single nucleotide polymorphisms (SNP), and DNA-based cancer diagnostics.