All-in-One Biochip for Infectious Disease Screening

描述(申请人提供)：在美国，每三秒就有人需要献血，这相当于每天大约使用4万品脱的血液。目前的血液筛查实验室测试非常耗时，必须对所有传染病“连续”进行。快速现场识别患有血液传播疾病的潜在献血者将有助于消除不必要的抽血、处理、储存和运输成本，并有助于防止进一步感染。该项目将开发一种一体化的传染病芯片(IDChip)技术，可以快速、灵敏地“同时”检测和识别多种(6-10)血液传播疾病。这款一体机IDChip包含了所有所需的试剂，因此从手指棒中加载非常小的体积是唯一需要手动参与的操作。这种一次性IDChip利用微制造和集成微流控技术，在圆盘封装中提供超灵敏的生化自动化。IDChip系统紧凑、便携，将有助于确保国家的血液供应不受传染病的影响。 在一期工程中，我们设计并制作了一个基于微流控的芯片，构建了一个实验室系统，并以三个靶点：乙肝表面抗原(HBs)、乙肝核心抗体(HBcAb)和丙型肝炎病毒抗体(HCVAb)对该系统进行了表征，进行了对照实验，并展示了检测能力。建议的IDChip系统不仅自动化了快速测试(&lt；25分钟)，而且还提供了出色的灵敏度。

DESCRIPTION (provided by applicant): Every three seconds someone in the United States requires donated blood, which correlates to approximately 40,000 pints of blood used each day. Current laboratory tests for blood screening are time consuming and must be performed "consecutively" for all infectious diseases. Rapid on-site identification of potential donors with blood borne diseases will help to eliminate needless blood draws, handling, storage, and transportation costs, and will help to prevent further infections. This project will develop an all-in-one infectious diseases chip (IDChip) technology that can rapidly, sensitively, and "simultaneously" detect and identify multiple (6-10) blood transmissible diseases. This all-in-one IDChip contains all required reagents, so loading a very small volume from a finger-stick is the only manual involvement required. This disposable IDChip utilizes microfabrication and integrated microfluidic technologies to provide ultrasensitive biochemistry automation in a disc package. The IDChip system is compact, portable, and will help to ensure that the nation's blood supply is free from infectious diseases. During the Phase I project, we have designed and fabricated a microfluidic based IDChip, constructed a laboratory system, and characterized the system with three targets: Hepatitis B Surface antigen (HBsAg), Hepatitis B Core Antibody (HBcAb), and Hepatitis C Virus Antibody (HCVAb), performed control experiments, and demonstrated the assay capabilities. The proposed IDChip system not only automated a rapid test (< 25 minutes), but also offers excellent sensitivity.