COPAS SELECT DROSOPHILA EMBRYO AND LARVA SORTER

本提案是为果蝇购买一个复杂对象参数分析仪和分选器（COPAS）。该仪器能够对大量转基因果蝇胚胎进行分选。这是一项最近可用的技术，可以进行各种功能基因组学研究，包括1)使用突变动物进行基因表达微阵列实验。2)利用突变果蝇胚胎进行染色质- dna共免疫沉淀实验；3)基于转基因动物GFP荧光激活或失活的新型遗传筛选。我们建议购买这台仪器，以供耶鲁调查人员直接使用。我们意识到这种新的仪器将允许果蝇基因组学的广泛应用，我们预计在第一年的运行中，最初的用户群体将需要仪器上的大部分可用时间。然而，就像大多数新仪器的情况一样，它允许以前在技术上极其困难或不可能的研究，我们预计耶鲁大学的用户群体将会增长。从一开始，我们将采用一个使用跟踪和管理系统，以最大限度地利用这一工具，为当地社区的研究人员谁使用果蝇在他们的研究。

This proposal is for the purchase of a Complex Object Parametric Analyzer and Sorter (COPAS) for Drosophila. This instrument is capable of sorting large quantities of genetically modified Drosophila embryos. This is a recently available technology that enables a variety of functional genomics investigations including 1) Gene expression microarray experiments using mutant animals. 2) Chromatin-DNA co- immunoprecipitation experiments using mutant Drosophila embryos, and 3) Novel genetic screens based on activation or deactivation of GFP fluorescence in transgenic animals. We propose to purchase this instrument for these immediate uses by Yale Investigators. We realize that this new instrumentation will allow for a wide variety of genomics applications in Drosophila, and we expect that for the first year of operation, the initial user group will demand most of the available time on the instrument. However, as it is the case for most new instrumentation that allows research that was previously exceedingly technically difficult or impossible, we expect that the user group will grow at Yale. From the onset, we will employ a system of usage tracking and management in order to maximize the utility of this instrument to the local community of investigators who use Drosophila in their research.