MONOAMINERGIC INFLUENCES ON VESTIBULO-SPINAL REFLEXES

单胺能对前庭脊髓反射的影响

• 批准号: 6642899
• 负责人: BILL J YATES
• 金额: $ 34.88万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6642899
• 关键词: afferent nerve; antidromic impulse; brain imaging /visualization /scanning; cats; dopamine beta monooxygenase; electromyography; electrophysiology; fluorescent dye /probe; immunocytochemistry; locus coeruleus; methyltransferase; neuroanatomy; neurochemistry; neuropharmacology; norepinephrine; proprioception /kinesthesia; tyrosine 3 monooxygenase; vestibular nuclei; vestibular pathway; vestibuloocular reflex

The primary goal of the proposed subproject is to examine the role of noradrenergic transmitters in the function of the vestibulo-spinal systems. Previous work has shown that there are noradrenergic inputs to portions of the vestibular nuclei, reticular formation, and spinal motor neurons, all of which are presumed to affect vestibulo-spinal reflexes. The first aim seeks to address the issue of whether regions of the vestibular nuclei that contain vestibulo-spinal projecting neurons receive noradrenergic inputs in cats. Immunohistochemical reactions of brainstem sections through the VNC will be performed for two precursor enzymes of norepinephrine (tyrosine hydroxylase and dopamine-beta-hydroxylase) and for the enzyme phenylethanol-N-methyltransferase as a control against epinephrine. The second aim attempts to determine which noradrenergic producing cell groups provide inputs to the vestibular nuclei and whether these cell groups in turn receive reciprocal input from the vestibular nuclei. Neural tracing studies in combination with immunohistochemistry for tyrosine-beta-hydroxylase will be performed to identify the regional projections between the noradrenergic cell groups and the vestibular nuclei. The third aim proposes to determine if the noradrenergic cells that project to the spinal cord, vestibular nuclei and/or reticular formation receive vestibular signals. Electrophysiological recordings from noradrenergic cells will be performed and the cell?s projections identified by antidromic invasion. The response to vestibular and neck stimulation will be examined. The fourth aim is to address the effects of noradrenergic transmitters upon the vestibulo-spinal reflexes. EMG recordings from the triceps muscle will be performed during vestibular stimulation, with application of norepinephrine, as well as noradrenergic agonists and antagonists into the lateral vestibular nucleus.

建议的子项目的主要目标是检查角色 去甲肾上腺素能递质在前庭-脊髓系统中的作用。 先前的工作表明，在大脑的某些部位有去甲肾上腺素的输入。 前庭核、网状结构和脊髓运动神经元，所有这些 被认为会影响前庭-脊髓反射。第一个目标是寻求 解决前庭核团区域是否包含 猫的前庭-脊髓投射神经元接受去甲肾上腺素能输入。 脑干切片通过VNC的免疫组织化学反应将是 对去甲肾上腺素的两种前体酶(酪氨酸羟基酶和 多巴胺-β-羟基酶)和苯乙醇-N-甲基转移酶 作为对肾上腺素的控制。第二个目标试图确定哪一个 去甲肾上腺素能产生细胞群为前庭核团提供输入 这些细胞群是否从前庭接受相互的输入 原子核。神经示踪结合免疫组织化学研究 将进行酪氨酸-β-羟基酶以确定区域投影 去甲肾上腺素能细胞群和前庭核团之间。第三个目标 建议确定投射到脊髓的去甲肾上腺素能细胞 脊髓、前庭核和/或网状结构接收前庭信号。 将进行去甲肾上腺素能细胞的电生理记录和 细胞？S通过逆行侵袭确定的投射。对以下问题的回应 将检查前庭和颈部刺激。第四个目标是解决 去甲肾上腺素能递质对前庭-脊髓反射的影响。 在前庭期间将进行三头肌肌电记录 刺激，应用去甲肾上腺素和去甲肾上腺素 激动剂和拮抗剂进入前庭外侧核。