MONOAMINERGIC INFLUENCES ON VESTIBULO-SPINAL REFLEXES

单胺能对前庭脊髓反射的影响

• 批准号: 6642899
• 负责人: BILL J YATES
• 金额: $ 34.88万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6642899
• 关键词: afferent nerve; antidromic impulse; brain imaging /visualization /scanning; cats; dopamine beta monooxygenase; electromyography; electrophysiology; fluorescent dye /probe; immunocytochemistry; locus coeruleus; methyltransferase; neuroanatomy; neurochemistry; neuropharmacology; norepinephrine; proprioception /kinesthesia; tyrosine 3 monooxygenase; vestibular nuclei; vestibular pathway; vestibuloocular reflex

The primary goal of the proposed subproject is to examine the role of noradrenergic transmitters in the function of the vestibulo-spinal systems. Previous work has shown that there are noradrenergic inputs to portions of the vestibular nuclei, reticular formation, and spinal motor neurons, all of which are presumed to affect vestibulo-spinal reflexes. The first aim seeks to address the issue of whether regions of the vestibular nuclei that contain vestibulo-spinal projecting neurons receive noradrenergic inputs in cats. Immunohistochemical reactions of brainstem sections through the VNC will be performed for two precursor enzymes of norepinephrine (tyrosine hydroxylase and dopamine-beta-hydroxylase) and for the enzyme phenylethanol-N-methyltransferase as a control against epinephrine. The second aim attempts to determine which noradrenergic producing cell groups provide inputs to the vestibular nuclei and whether these cell groups in turn receive reciprocal input from the vestibular nuclei. Neural tracing studies in combination with immunohistochemistry for tyrosine-beta-hydroxylase will be performed to identify the regional projections between the noradrenergic cell groups and the vestibular nuclei. The third aim proposes to determine if the noradrenergic cells that project to the spinal cord, vestibular nuclei and/or reticular formation receive vestibular signals. Electrophysiological recordings from noradrenergic cells will be performed and the cell?s projections identified by antidromic invasion. The response to vestibular and neck stimulation will be examined. The fourth aim is to address the effects of noradrenergic transmitters upon the vestibulo-spinal reflexes. EMG recordings from the triceps muscle will be performed during vestibular stimulation, with application of norepinephrine, as well as noradrenergic agonists and antagonists into the lateral vestibular nucleus.

提议的副本的主要目标是检查角色 北肾上腺素能发射器在前庭脊柱系统的功能中。 以前的工作表明，部分有非肾上腺素能量 前庭核，网状形成和脊柱运动神经元，所有这些 假定会影响前庭脊柱反射。第一个目标试图 解决了是否包含前庭核区域的问题 前庭脊柱突出神经元在猫中接受去甲肾上腺素能的输入。 通过VNC的脑干切片的免疫组织化学反应将是 对去甲肾上腺素的两个前体酶（酪氨酸羟化酶和 多巴胺β-羟化酶）和用于苯乙醇-N-甲基转移酶的酶 作为对肾上腺素的控制。第二个目标试图确定哪个 去肾上腺素能细胞组为前庭核提供了输入， 这些细胞组是否依次从前庭获得相互输入 核。神经追踪研究与免疫组织化学结合 将执行酪氨酸β-羟化酶以识别区域预测 在去甲肾上腺素能细胞组和前庭核之间。第三个目标 建议确定投射到脊柱的去甲肾上腺素能细胞 绳索，前庭核和/或网状形成接收前庭信号。 将执行来自去甲肾上腺素细胞的电生理记录，并 通过抗体入侵确定的细胞投影。对 将检查前庭和颈部刺激。第四个目标是解决 去甲肾上腺素能发射器对前庭脊柱反射的影响。 前庭中将进行三头肌的EMG记录 使用去甲肾上腺素以及去甲肾上腺素的刺激 激动剂和拮抗剂进入前庭核。