CORE--MURINE AND HUMAN STEM CELL

描述（直接从应用程序中获取）：此目标 方案项目是 研究造血干细胞的细胞和分子生物学。 为了实现计划的目标？的个别项目，它是 简单地说，一个可靠的造血干细胞来源， 需要祖细胞。还需要培养系统， 允许这些细胞的储存、测定和扩增。的目标 干/祖细胞核心是为了满足这些需求，提供 节目？的研究人员与良好表征的小鼠和人类 造血细胞来自各种细胞来源，并在各种 分化的水平。为了支持这一目标， 具体目标如下：1）优化现有的 分离和储存人和鼠干/祖细胞的方法 细胞，以及2）建立并提供长期和短期培养物 用于分离细胞的发育潜力的生物测定系统， 包括免疫缺陷小鼠的移植模型。

Description (taken directly from the application): The goal of this program projects is to study the cellular and molecular biology of hematopoietic stem cells. To accomplish the goals of the program?s individual projects, it is straightforward that a reliable source of hematopoietic stem and progenitor cells is required. Also required are culture systems which allow for the storage, assay, and expansion of these cells. The goal of Stem/Progenitor Cell Core is to meet these needs by supplying the program?s investigators with well characterized murine and human hematopoietic cells from a variety of cellular sources and at various levels of differentiation. To support this goal the core has developed two specific aims which are as follows: 1) Optimize presently established methods for isolation and storage of human and murine stem/progenitor cells, and 2) Establish, and make available long and short term culture systems for bioassay of the developmental potential of isolated cells, including transplantation models into immunodeficient mice.