STRUCTURAL STUDIES OF CARBAMOYL PHOSPHATE SYNTHETASE

氨基甲酰磷酸合成酶的结构研究

• 批准号: 6586546
• 负责人: IVAN RAYMENT
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586546
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

It is planned to determine the three-dimensional structure of carbamoyl phosphate from Escherichia coli, a high energy intermediate used in several biosynthetic pathways. This enzyme catalyzes the formation of carbamoyl phosphate from glutamine, bicarbonate, and ATP, and is one of the first steps in the biosynthesis of pyrimidines. Conditions for flash-freezing of the crystal have been established and a search for heavy atom derivatives has been initiated.

计划确定三维结构 来自大肠杆菌的磷酸甲甲酰磷酸盐，高能中间体 用于几种生物合成途径。 这种酶催化了 从谷氨酰胺，碳酸氢盐和ATP形成磷酸甲酰磷酸 并且是嘧啶生物合成的第一步之一。 已经建立了晶体闪存的条件，并且 启动了对沉重的原子衍生物的搜索。