CRYSTALLOGRAPHIC STUDIES OF MYOSIN SUBFRAGMENT 1

肌球蛋白亚片段 1 的晶体学研究

• 批准号: 6658537
• 负责人: IVAN RAYMENT
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658537
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; musculoskeletal system; proteins; radiography; structural biology

The objective of this research is to improve our understanding of the molecular basis of muscle contraction by continuing our studies of the three-dimensional structure of myosin subfragment-1 using single-crystal x-ray diffraction. Myosin is the major protein in muscle, in which it plays both a structural and enzymatic role. The myosin rod forms the backbone of the thick filament, whereas the myosin head is responsible for the generation of movement through the hydrolysis of ATP and its interaction with actin. The long term goal is to characterize all of the conformational intermediates in the contractile cycle which should then form the basis of a molecular mechanism of myosin-based motility.

这项研究的目的是提高我们对 通过继续研究肌肉收缩的分子基础 肌球蛋白亚段-1的三维结构 单晶X射线衍射仪。肌球蛋白是人体内的主要蛋白质 肌肉，在其中它既起着结构作用又起着酶的作用。这个 肌球蛋白棒构成粗丝的主干，而肌球蛋白棒 肌球蛋白头部负责通过 ATP的水解性及其与肌动蛋白的相互作用。长期目标 是表征所有的构象中间体 收缩循环，然后形成一个分子的基础 基于肌球蛋白的运动机制。