CRYSTALLOGRAPHIC STUDIES OF REP HELICASE IN COMPLEX W/ SINGLE STRANDED DNA

单链 DNA 复合物 REP 解旋酶的晶体学研究

• 批准号: 6586531
• 负责人: SRINIVASAN RAGHUNATHAN
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586531
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

The focus of our structural studies is the E. coli Rep helicase. The crystals are tetragonal and diffract to a resolution of 4 E. We hope to extend the resolution of diffraction so that structure determination may be achieved at moderate atomic resolution by multiple isomorphous replacement technique. This study is the first attempt to solve the structure of any helicase using X-ray crystallography. The crystal structure determination of Rep helicase will contribute significantly to our understanding of the mechanism by which DNA is unwound.

我们结构研究的重点是E. coli Rep解旋酶。 晶体为四重态，分辨率为4E。我们 希望能扩展衍射的分辨率， 可以通过以下步骤以中等原子分辨率实现确定 多重同晶置换技术 这项研究首次 试图用X射线解析解旋酶的结构 结晶学 Rep解旋酶的晶体结构测定 将大大有助于我们对该机制的理解， 哪一个DNA被解开了