CRYSTALLOGRAPHIC STUDIES OF REP HELICASE IN COMPLEX W/ SINGLE STRANDED DNA

我们结构研究的重点是大肠杆菌Rep解旋酶。 晶体为四方晶体，衍射率为4E。 希望扩大衍射的分辨率，使结构 可以通过以下方式在中等原子分辨率下进行测定 多重同构置换技术。这项研究是第一次 尝试用X射线解决任何解旋酶的结构 结晶学。Rep解旋酶的晶体结构测定 将极大地促进我们对这一机制的理解 哪个DNA被解开了。

The focus of our structural studies is the E. coli Rep helicase. The crystals are tetragonal and diffract to a resolution of 4 E. We hope to extend the resolution of diffraction so that structure determination may be achieved at moderate atomic resolution by multiple isomorphous replacement technique. This study is the first attempt to solve the structure of any helicase using X-ray crystallography. The crystal structure determination of Rep helicase will contribute significantly to our understanding of the mechanism by which DNA is unwound.