XRAY DIFFRACTION OF CLATHRIN HUB CRYSTALS

包衣前庭蛋白(CCV)介导的内吞作用 受体结合的营养物质、激素和蛋白质 降解，并调节这些配体的生理水平。CCV 调解跨高尔基网络中的分拣过程 溶酶体和分泌物颗粒的生物发生。我们已经表达了， 纯化并结晶了含有以下成分的笼蛋白HUB/LCB复合体 重链和神经性LCB轻链的残基1074-1675。 研究表明，HUB/LCB复合体包含所有信息 组装所需的。对结构的阐明将揭示 组装过程的分子机理。我们拥有的水晶是 太小，无法在旋转的阳极上获得足够高的分辨率数据 用于结构确定的生成器。我们想要得到 在同步辐射下达到3E分辨率或更高分辨率的衍射 并且，收集完整的原生数据集。我们已经确定了 对马赛克影响不大的冻结条件 衍射图的形状。

Clathrin-coated vesticles (CCV) mediate endocytosis of receptor-bound nutrients, hormones and proteins destined for degradation, and regulate physiological levels of these ligands. CCV mediates sorting processes in the trans-Golgi network required for biogenesis of lysosomes and secretary granules. We have expressed, purified and crystallized the clathrin hub/LCb complex which contains residues 1074-1675 of the heavy chain and neuronal Lcb light chain. Studies demonstrated that the hub/LCb complex contains all information needed for assembly. Elucidation of the structure will reveal the molecular mechanism for assembly process. The crystals we have are too small to get high enough resolution data on a rotating anode generator for a structure determination. We would like to get diffraction to 3 E resolution or beyond at a synchrotron radiation and, collect a complete native data set. We have determined the conditions to freeze the crystals with minor effect on the mosaicity of the diffraction pattern.