CORE--NUCLEIC ACID RESEARCH

核酸研究设施（NARF），前身为核酸测序和合成资源（NASSR），成立于1986年秋季，自那时以来一直是梅西癌症的重要组成部分。该设施现在支持自动DNA测序、DNA合成和纯化、真实的时间PCR和DNA微阵列。化验所继续保持正常及修饰寡核苷酸合成及DNA定序的稳定增长。自上次提交以来，NARF增加了两项新的核心服务：真实的时间PCR和DNA微阵列。真实的时间PCR已经非常好地接受和活动不断增加，在略多于12个月，我们已经提供了服务。该实验室于2000年底确定了购买微阵列仪器的资金。从那时起，我们已经购买并安装了Affychip基因芯片/TM系统和斑点阵列系统，包括BioRobotics TAS II斑点仪和Packard ScanArray 5000扫描仪。还获得并安装了辅助仪器，包括一组96和384孔热循环仪、液体处理设备，以及高通量仪器，包括一组96和384孔热循环仪、液体处理设备和高通量cDNA克隆模板制备。与这些发展并行的是，NARF的人员队伍扩大到包括我们的测序核心经理，他在俄克拉荷马州大学基因组中心接受过培训，是一名博士一位训练有素的助理教授负责监督MicroArraying Facility，我们的MS培训DNA合成和RT PCR Core的DNA经理，以及两名BS培训的技术人员。随着微阵列和RT PCR仪器的收购，两个额外的小实验室被添加到包括NARF的实验室套件中，该实验室套件现在包括四个主要实验室，两个办公室，一个专用于GeneChip/TM系统的小实验室和一个数据分析资源。

The Nucleic Acids Research Facilities (NARF), formerly the Nucleic Acids Sequencing and Synthesis Resource (NASSR) was established in fall of 1986, and has been an important component of the Massey Cancer since that time. The facility now supports automated DNA sequencing, DNA synthesis and purification, Real Time PCR, and DNA microarraying. The Laboratory has continued to maintain a steadily increasing throughput of normal and modified oligonucleotide synthesis and DNA sequencing. Since the last submission, the NARF has added two new core services: Real Time PCR and DNA MicroArraying. Real Time PCR has been extremely well received and activity has increased continuously over the slightly more than 12 months that we have offered the service. The laboratory identified funds to acquire the microarraying instrumentation in late 2000. Since that time, we have purchased and installed the Affymetrix GeneChip/TM System and a Spotted Array System consisting of a BioRobotics TAS II Spotter and a Packard ScanArray 5000 Scanner. Ancillary instrumentation, including a bank of 96 and 384 well thermocyclers, liquid handling equipment, and a high throughput including a bank of 96 and 384 well thermocyclers, liquid handling equipment, and a high throughput cDNA clone template preparation was also acquired and installed. In parallel with these developments, the personnel of the NARF expanded to include our Sequencing Core Manager who trained in the University of Oklahoma Genome Center, a Ph.D. trained assistant professor to oversee the MicroArraying Facility, our MS trained DNA manager of the DNA synthesis and RT PCR Core, and two BS trained technicians. With the acquisition of the Microarraying and RT PCR instrumentation, two additional small labs were added to the suite of labs that comprise the NARF, which now consists of four main labs, two offices, a small lb dedicated to the GeneChip/TM System, and a data analysis resource.