FLUORIDE AND OTHER FACTORS IN CHILDHOOD BONE DEVELOPMENT

氟化物和儿童骨骼发育中的其他因素

• 批准号: 6516490
• 负责人: STEVEN M. LEVY
• 金额: $ 48.42万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6516490
• 关键词: alleles; body height; body weight; bone density; bone development; child physical development; clinical research; developmental genetics; developmental nutrition; dietary calcium; exercise; fluoride ion; gender difference; human subject; longitudinal human study; middle childhood (6-11); nutrition related tag; osteocalcin; osteonectin; osteopontin; photon absorptiometry; polymerase chain reaction; preschool child (1-5); vitamin D; vitamin D receptors

Recent studies have demonstrated a link between low peak bone mass in early adulthood and osteoporosis later in life. Studies have indicated that calcium (Ca) intake and exercise patterns may be associated with bone mass and density in older children and adults, and that fluoride (F) may affect adult bone. However, few studies included younger children and none have examined the effects of F or Ca intake and genetic factors on bone mass/density in this age group. The goals of this project are to characterize biologic variation in bone development in healthy young children, and to understand how nutritional, behavioral, genetic and other factors interact to influence bone accretion in childhood, leading to attainment of peak bone mass in early adulthood. A cohort of 400 children already participating in a NIDR-funded longitudinal study of fluoride intake and dental fluorosis (NIDR RO1-DE09551) will be studied. Detailed information about daily intakes of F, Ca, Vitamin D, and other nutrients, and medical history is already available for each child since birth. We will continue to collect these data at 4 month intervals, and will collect exercise data for each child. To assess acquisition of bone mass over time, bone density measurements of the lumbar spine, hip, and whole body will be made, using dual-energy x-ray absorptiometry at age 4-5 years, and again at 7-8 years. Allelic variation of 6 bone-related genes will be determined by PCR-based assays, with whole blood DNA as template. Bivariate and multivariate analyses will be used to identify major modifiers of bone mass/density and bone accretion, and to examine potential interactions among these factors as they relate to bone acquisition. This study offers a unique opportunity to provide valuable insight into bone development in children, using a well-organized existing cohort for which extensive data are already available. Our results could serve as a basis for future recommendations regarding modifiable factors (such as nutrient intake and exercise) for enhancement of bone mass/density, and thereby may have important implications for prevention of adult-onset conditions such as osteoporosis.

最近的研究表明， 成年早期和以后的骨质疏松症。 研究表明 钙（Ca）摄入量和运动模式可能与 年龄较大的儿童和成人的骨量和密度， (F)可能影响成人骨骼。 然而，很少有研究包括年轻人， 儿童和没有人检查了F或Ca摄入量的影响， 遗传因素对该年龄组骨量/密度的影响。 这个项目的目标是表征骨的生物学变异 在健康的幼儿发展，并了解如何 营养、行为、遗传和其他因素相互作用， 儿童时期的骨质增生， 成年早期 一个400名儿童的队列已经参加了一个 NIDR资助的氟摄入量与氟斑牙的纵向研究 (NIDR RO 1-DE 09551）进行研究。 每日详细信息 F、Ca、维生素D和其他营养素的摄入量以及病史 每个孩子从出生起就可以使用。 我们将继续 每隔4个月收集这些数据，并将收集运动数据 为每个孩子。 为了评估骨量随时间的获得，骨 腰椎、髋关节和全身的密度测量将 在4-5岁时使用双能X射线吸收测定法， 7-8岁。 6个骨相关基因的等位基因变异将是 通过基于PCR的测定，以全血DNA为模板。 双变量和多变量分析将用于确定主要 骨质量/密度和骨增生的修饰剂，并检查 这些因素之间的潜在相互作用，因为它们与骨有关 采集 这项研究提供了一个独特的机会， 深入了解儿童骨骼发育，使用组织良好的 现有的队列已经有了广泛的数据。 我们 研究结果可作为今后就下列问题提出建议的基础： 可改变的因素（如营养摄入和运动）， 骨质量/密度的增强，从而可能具有重要的 对预防成人发病的影响， 骨质疏松