Vitrified Immobilized Proteins and Ligands

玻璃化固定化蛋白质和配体

• 批准号: 6642577
• 负责人: GARY M NISHIOKA
• 金额: $ 36.79万
• 依托单位: H AND N INSTRUMENTS, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6642577
• 关键词: affinity chromatography; biomedical equipment development; biotechnology; enzyme linked immunosorbent assay; immobilized enzymes; ligands; microarray technology; peptide chemical synthesis; preservation; protein purification; protein structure function; technology /technique development

DESCRIPTION (provided by applicant): The overall objective of this research is to develop a new process and laboratory instrument capable of synthesizing microspots of vitrified immobilized proteins and ligands. Immobilized proteins and ligands stored in a vitrified (glassy) sugar matrix would be well preserved at room and even elevated temperatures. The ability to create such microspots could generate significant improvements in performance, cost, convenience, and storage requirements for applications requiring immobilized proteins and ligands. Examples of applications that could be improved include immunoassays, syntheses using immobilized enzymes, purifications involving affinity chromatography, and sensors using immobilized antibodies, enzymes, and ligands.

描述（由申请人提供）：本研究的总体目标是开发一种新的工艺和实验室仪器，能够合成玻璃化固定蛋白质和配体的微点。固定的蛋白质和配体储存在玻璃化的糖基质中，在室温甚至高温下都能很好地保存。对于需要固定蛋白质和配体的应用来说，制造这种微点的能力可以在性能、成本、便利性和存储要求方面产生重大改进。可以改进的应用实例包括免疫测定、使用固定化酶的合成、涉及亲和层析的纯化以及使用固定化抗体、酶和配体的传感器。