ANTIGEN SCANNING ON COMBINATORIAL ARRAYS

组合阵列上的抗原扫描

• 批准号: 2714934
• 负责人: GARY M NISHIOKA
• 金额: $ 10万
• 依托单位: H AND N INSTRUMENTS, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2714934
• 关键词: antigens; atomic force microscopy; chemical registry /resource; chemical synthesis; drug screening /evaluation; method development; peptide library; pharmacology; physical chemical interaction

DESCRIPTION: (Adapted from the applicant's abstract) Combinatorial techniques to synthesize a host of new compounds are being rapidly developed, leading to new candidates for therapeutic and diagnostic treatments. In contrast, the development of techniques to exploit these combinatorial libraries has been relatively static. In every method the recognition step relies on strong adsorption of the antigen of interest to one member of the library. This recognition step could overlook moderate interactions or subtle differences that could provide important clues for further screening. In addition, this step limits the use of mixed libraries in screening protocols. They propose to develop a new recognition step that will circumvent the above difficulties. The method is applicable to combinatorial libraries on smooth, flat surfaces. The method involves the direct measurement of the interaction of the antigen with each library member using atomic force microscopy. If successful, the proposed method would provide a quantitative measure of the interaction of each library member with an antigen of interest. This would be a new technique to characterize antigens. The method would also enhance the use of mixed libraries in screening protocols. This could result in highly efficient screening protocols that require a lower number of library members. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：（改编自申请人的摘要） 合成许多新化合物的技术正在迅速发展， 开发，导致新的候选人的治疗和诊断 治疗。 相比之下，开发利用这些技术 组合文库一直相对静态。 在每种方法中， 识别步骤依赖于感兴趣的抗原的强吸附， 图书馆的一员。 这一识别步骤可能会忽略中度 相互作用或微妙的差异，可以提供重要的线索， 进一步筛选。 此外，该步骤限制了混合的使用。 筛选方案中的文库。 他们建议开发一种新的识别步骤， 在困难之上。 该方法适用于组合文库 在光滑平坦的表面上 该方法涉及直接测量 使用原子力的抗原与每个文库成员的相互作用 显微镜 如果成功，所提出的方法将提供一个 定量测量每个文库成员与一个 目标抗原。 这将是一种新的技术， 抗原 该方法还将加强混合库的使用， 筛选方案。这可能导致高效的筛选 需要较少数量的库成员的协议。 拟议商业应用：不可用