Sensitive Detection of Urinary Biomarkers of Renal Injury

描述(由申请人提供)：如果成功，这个计划将导致一个分析芯片，收集，稳定，并准确地量化一套指示肾脏损伤的生物标志物。它的灵敏度和准确性可能使其能够检测到缓慢发生的肾脏损害。它的多分析能力可以区分不同的肾脏损害机制。它应该适用于自动化分析，因此可用于临床环境。 公共卫生相关性：对于肾脏疾病患者、接受肾移植的患者以及接受药物治疗的患者，特别是可能导致肾脏损伤的新药，早期诊断肾脏损伤的必要性非常重要。传统的生物标志物不能检测出早期的肾脏损害。出于这个原因，人们对寻找新的肾损伤生物标志物非常感兴趣和活跃。开发使用新生物标记物的新化验模式的活动较少。我们建议开发一种这样的分析方法。我们建议开发一种诊断芯片，能够收集、稳定并高度敏感和准确地定量一套指示肾脏损害的生物标志物。

DESCRIPTION (provided by applicant): If successful, this program will result in an assay chip that collects, stabilizes, and accurately quantifies a suite of biomarkers indicative of renal injury. Its sensitivity and accuracy may enable it to detect slowly occurring renal damage. Its multianalyte capability could distinguish different mechanisms of renal damage. It should be adaptable to automated assays and hence usable in a clinical setting. PUBLIC HEALTH RELEVANCE: The need for an early diagnosis of renal injury is important for patients with renal disease, for those undergoing kidney transplantation, and for patients undergoing drug therapy, especially new drugs that may cause renal damage. Traditional biomarkers are not capable of detecting early stages of renal damage. For this reason there is high interest and activity in the search for new biomarkers of renal damage. There is less activity in developing new assay formats that use new biomarkers. We propose to develop a such an assay. We propose developing a diagnostic chip capable of collection, stabilization, and highly sensitive and accurate quantification of a suite of biomarkers indicative of renal damage.