Vitrified Immobilized Proteins and Ligands

描述(申请人提供)：本研究的总体目标是 开发一种可合成的新工艺和实验室仪器 玻璃化固定化蛋白质和配体的微斑点。固定化蛋白 而储存在玻璃化(玻璃)糖基质中的配体将被很好地保存 在室温下，甚至在高温下。创造这种微斑点的能力 可以在性能、成本、便利性和 需要固定化蛋白质的应用的存储要求和 配基。可以改进的应用的例子包括免疫分析， 使用固定化酶的合成，涉及亲和力的纯化 层析，以及使用固定化抗体、酶和配体的传感器。 我们建议使用名为SCAMP的微型喷墨打印机来创建玻璃化 微斑点。我们开发了SCAMP作为打印多肽的实验室仪器 微阵列。我们建议修改SCAMP以打印蛋白质/糖溶液，以及 确定产生玻璃化微斑的条件。微斑点 最初打印的尺寸是喷墨打印机的典型尺寸：100 皮升体积，形成直径60-100微米的斑点。这个 还将确定使用这项技术的优点和缺点。 建议的商业应用： 提出的方法可以改进免疫分析，使用固定化酶进行合成，使用亲和层析进行纯化，以及使用固定化抗体、酶和配体进行传感器。

DESCRIPTION (provided by applicant): The overall objective of this research is to develop a new process and laboratory instrument capable of synthesizing microspots of vitrified immobilized proteins and ligands. Immobilized proteins and ligands stored in a vitrified (glassy) sugar matrix would be well preserved at room and even elevated temperatures. The ability to create such microspots could generate significant improvements in performance, cost, convenience, and storage requirements for applications requiring immobilized proteins and ligands. Examples of applications that could be improved include immunoassays, syntheses using immobilized enzymes, purifications involving affinity chromatography, and sensors using immobilized antibodies, enzymes, and ligands. We propose to use a microjet printer, designated SCAMP, to create vitrified microspots. We developed SCAMP as a laboratory instrument to print peptide microarrays. We propose to modify SCAMP to print protein/sugar solutions, and determine the conditions that yield vitrified microspots. The microspots printed would initially be of dimensions typical for an ink jet printer: 100 picoliters volume, creating a spot 60 - 100 micrometers in diameter. The advantages and drawbacks in using this technology will also be determined. PROPOSED COMMERCIAL APPLICATIONS: The proposed method could lead to improved immunoassays, syntheses using immobilized enzymes, purifications involving affinity chromatography, and sensors using immobilized antibodies, enzymes, and ligands.