Integrated Standardized Express. Analytical Tools
综合标准化快递。
基本信息
- 批准号:6688130
- 负责人:
- 金额:$ 16.4万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2003
- 资助国家:美国
- 起止时间:2003-09-30 至 2004-09-29
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant): The DNA databases have been a paradigm shift in science, where the end result of large-scale data generation is not so much publications, but rather a public web-based resource (genome browsers) with integrated component databases having high accuracy and sensitivity. The genome browsers are rapidly becoming one of the major tools of the bio-medical research community, and are dramatically expediting scientific discovery in genetics and biology in general. The DNA databases are relatively "static" in the sense that there are relatively few variables to be considered; e.g. "time", and "amplitude" are generally not variables. Genome-wide information on RNA and protein are much more dynamic, with many more variables, most of which are dynamic in nature. Current efforts at providing public access databases in RNA expression profile data have been largely limited to "data repositories", with only limited query tools and cross-experiment investigations possible. A major limitation of development of more powerful expression profile data warehouses and analytical tools has been the lack of a standard experimental platform, and considerable difficulty in signal/noise interpretation. We have recently circumvented many of these limitations, and have made public the first web-Oracle integrated expression-profiling database. We have developed strict QC/SOP protocols using the data-intensive and accurate Affymetrix platform, have developed a web-queried data warehouse with some analytical tools, and have written and implemented automated data conversion protocols for web publication to both our web server and NCBI GEO. Indeed, about 50% of all expression profile data in the popular UCSC genome browsers is from our integrated internal/web LIMS Oracle data warehouse (5,350 scans currently stored and analyzed). Here, our R21 request is to develop innovative web-based analytical tools that allow the casual user to query their gene of interest in any or all of the arrays in our data warehouse (human, mouse, rat; >140 projects, with many time series data). The major benchmark of our R21 phase will be the demonstration of widespread popularity of this innovative tool, by quantitation of data queries and user feedback. The R33 phase will develop, implementation of remote data entry and QC checks by other core facilities, expansion of the warehouse to > 10,000 vertebrate profiles, and development of a suite of innovative user analysis and visualization tools for the web data warehouse.
描述(申请人提供):DNA数据库是科学中的一种范式转变,大规模数据生成的最终结果不是那么多的出版物,而是一个公共的基于网络的资源(基因组浏览器),具有集成的组件数据库,具有高准确性和敏感性。基因组浏览器正在迅速成为生物医学研究社区的主要工具之一,并极大地加速了遗传学和生物学的总体科学发现。DNA数据库相对“静态”,因为需要考虑的变量相对较少;例如,“时间”和“幅度”通常不是变量。关于RNA和蛋白质的全基因组信息要动态得多,有更多的变量,其中大多数是动态的。目前在RNA表达谱数据中提供公共访问数据库的努力在很大程度上局限于“数据仓库”,只有有限的查询工具和交叉实验调查可能。开发更强大的表达谱数据仓库和分析工具的主要限制是缺乏标准的实验平台,并且在信号/噪声解释方面存在相当大的困难。我们最近绕过了许多这些限制,并公开了第一个Web-Oracle集成的表达分析数据库。我们利用数据密集型和精确的Affymetrix平台制定了严格的QC/SOP协议,开发了带有一些分析工具的网络查询数据仓库,并编写和实施了网络发布到我们的网络服务器和NCBI GEO的自动数据转换协议。事实上,在流行的UCSC基因组浏览器中,大约50%的表达谱数据来自我们集成的内部/网络LIMS Oracle数据仓库(目前存储和分析了5,350次扫描)。在这里,我们的R21要求开发创新的基于Web的分析工具,允许临时用户在我们的数据仓库(人、鼠、鼠;>;140项目,具有许多时间序列数据)中的任何或所有阵列中查询他们感兴趣的基因。我们R21阶段的主要基准将是通过量化数据查询和用户反馈来展示这一创新工具的广泛受欢迎程度。R33阶段将开发、实施其他核心设施的远程数据录入和质量控制检查,将数据仓库扩大到10,000个脊椎动物档案,并为网络数据仓库开发一套创新的用户分析和可视化工具。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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ERIC P. HOFFMAN其他文献
Proteolytic fragment or new gene product?
蛋白水解片段还是新基因产物?
- DOI:
10.1038/336210a0 - 发表时间:
1988-11-01 - 期刊:
- 影响因子:48.500
- 作者:
ERIC P. HOFFMAN;LOUIS M. KUNKEL;ROBERT H. BROWN - 通讯作者:
ROBERT H. BROWN
ERIC P. HOFFMAN的其他文献
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{{ truncateString('ERIC P. HOFFMAN', 18)}}的其他基金
Clinical Trial Readiness for Monitoring Muscle Inflammation in Duchenne Muscular Dystrophy
监测杜氏肌营养不良症肌肉炎症的临床试验准备
- 批准号:
10725465 - 财政年份:2023
- 资助金额:
$ 16.4万 - 项目类别:
Commercialization Readiness Pilot (CRP) to maximize vamorolone international labeling and sales
商业化准备试点 (CRP),以最大限度地提高瓦莫洛龙的国际标签和销售
- 批准号:
10200153 - 财政年份:2016
- 资助金额:
$ 16.4万 - 项目类别:
K12 Career Development Program: Omics of Pediatric Lung Diseases in DC
K12 职业发展计划:华盛顿特区小儿肺部疾病组学
- 批准号:
8857246 - 财政年份:2013
- 资助金额:
$ 16.4万 - 项目类别:
K12 Career Development Program: Omics of Pediatric Lung Diseases in DC
K12 职业发展计划:华盛顿特区小儿肺部疾病组学
- 批准号:
8575197 - 财政年份:2013
- 资助金额:
$ 16.4万 - 项目类别:
K12 Career Development Program: Omics of Pediatric Lung Diseases in DC
K12 职业发展计划:华盛顿特区小儿肺部疾病组学
- 批准号:
8722615 - 财政年份:2013
- 资助金额:
$ 16.4万 - 项目类别:
Center for Research Translation of Systemic Exon-skipping in Muscular Dystrophy
肌营养不良症系统性外显子跳跃研究转化中心
- 批准号:
8544772 - 财政年份:2011
- 资助金额:
$ 16.4万 - 项目类别:
Center for Research Translation of Systemic Exon-skipping in Muscular Dystrophy
肌营养不良症系统性外显子跳跃研究转化中心
- 批准号:
8330812 - 财政年份:2011
- 资助金额:
$ 16.4万 - 项目类别:
Center for Research Translation of Systemic Exon-skipping in Muscular Dystrophy
肌营养不良症系统性外显子跳跃研究转化中心
- 批准号:
8734214 - 财政年份:2011
- 资助金额:
$ 16.4万 - 项目类别:
Center for Research Translation of Systemic Exon-skipping in Muscular Dystrophy
肌营养不良症系统性外显子跳跃研究转化中心
- 批准号:
8090706 - 财政年份:2011
- 资助金额:
$ 16.4万 - 项目类别:
AN EXERCISE INTERVENTION IN INSULIN-RESISTANT MINORITY ADOLESCENTS
对胰岛素抵抗的少数青少年的运动干预
- 批准号:
8167332 - 财政年份:2010
- 资助金额:
$ 16.4万 - 项目类别: