Integrated Molecular Core for Rehabilitation Medicine

康复医学综合分子核心

• 批准号: 8053664
• 负责人: ERIC P. HOFFMAN
• 金额: $ 1.07万
• 依托单位: CHILDREN'S RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8053664
• 关键词: Adherence; American; Amino Acids; Area; Arts; Bioinformatics; Biological Assay; Child; Chromatography; Clinical; Clinical Data; Clinical Protocols; Clinical Trials; Clinical Trials Network; Collaborations; Computer software; Congresses; Consultations; Core Facility; Cultured Cells; DNA; DNA Fingerprinting; Data; Data Analyses; Data Files; Data Set; Databases; Development; Diagnostic; District of Columbia; Educational Intervention; Educational workshop; Ensure; Equipment; Exercise; Exons; Experimental Designs; Fibroblasts; Funding; Future; Gel; Gene Mutation; Generations; Genetic; Genetic Medicine; Genetic Research; Genome; Genomics; Genotype; Goals; Health Services Research; High Pressure Liquid Chromatography; Human; Image; Individual; Institution; Internet; Intervention Studies; Ions; Knowledge; Label; Laboratories; Licensing; Magnetic Resonance Imaging; Mass Spectrum Analysis; Measurement; Medical center; Medicine; Mental Retardation and Developmental Disabilities Research Centers; Messenger RNA; Metabolic; Methodology; Methods; Mining; Molecular; Molecular Profiling; Mutation; National Institute of Child Health and Human Development; Oligonucleotides; Patients; Phase; Post-Translational Protein Processing; Principal Investigator; Professional counselor; Proteins; Proteomics; Protocols documentation; Public Domains; Publications; Rehabilitation Research; Rehabilitation therapy; Research; Research Infrastructure; Research Personnel; Resource Development; Resources; Reverse Transcriptase Polymerase Chain Reaction; Robot; Robotics; Sampling; Scientist; Screening procedure; Services; Shotguns; Single Nucleotide Polymorphism; Site; Stable Isotope Labeling; Structure; Technology; Testing; Time; Training; Training Programs; Validation; Vision; Work; Writing; base; comparative; computerized data processing; data exchange; database design; design; experience; genetic linkage analysis; genome-wide; human subject; improved; liquid chromatography mass spectrometry; mass spectrometer; member; multidisciplinary; nano; neuromuscular; programs; small molecule; software development; strength training; technique development; therapy development; tumor; volunteer; web interface; web site

DESCRIPTION (provided by applicant): We describe a proposed and integrated molecular and public access core in support of rehabilitation research within the NCMRR network. The proposed Core is a pre-existing collaborative group of experts in genomewide DNA, mRNA, and protein analyses at the Research Center for Genetic Medicine, Children's National Medical Center, Washington DC. The goal of the proposed Analytical and Databasing Core is to provide access to collateral expertise in molecular/genomic technologies and bioinformatics that is particularly relevant to current opportunities in medical rehabilitation research. We propose a program of training, research resources, and collaborative opportunities in highly parallel and/or high throughput DNA, mRNA, proteomics methods, with additional support in web database design and implementation, with integration of molecular and clinical data. Both private and public interfaces will be designed and supported. This will be accomplished through a combination of didactic interactions (workshops, courses, written material, and websites), consultations, sabbatical opportunities, and pilot funding. In addition, we propose two intramural activities related to technique development, adaptation, and validation: integration of volumetric MRI, SNP data, and clinical data for 1,200 volunteers in a longitudinal resistance training intervention, and development of QC/SOP and quantitative analysis methods for proteomic profiling using metabolic labeling. The proposed Core will support DNA analyses (mutation studies, SNP discovery, SNP genotyping, linkage analyses, Aim 1); mRNA analyses (microarray mRNA profiling, quantitative RT-PCR, Aim 2); and proteomics analyses (large and small molecule high throughput mass spectrometry, comparative proteomic profiling, Aim 3). $3.2 million of state-of-the-art equipment is available within the proposed core for support of all types of genomics and proteomics studies undertaken by NCMRR investigators. Pre-existing NICHD- and NCRR-funded core facilities that would be synergistic with the proposed Analytical Core include a MRDDRC Genetics Core, MRDDRC Proteomics Core, CRC Genetics Core, and CHDRCA Genetics Training Core.

描述（由申请人提供）：我们描述了一个提议的和集成的分子和公共访问核心，以支持NCMRR网络中的康复研究。拟议的核心是华盛顿特区儿童国家医学中心研究中心，在全基因组DNA，mRNA和蛋白质分析方面的专家组成的合作小组。拟议的分析和数据库核心的目的是提供与分子/基因组技术和生物信息学方面的附带专业知识，这与当前医学康复研究的机会特别相关。我们提出了一个培训，研究资源和协作机会的计划，以高度平行和/或高吞吐量DNA，mRNA，蛋白质组学方法，并在Web数据库设计和实施中提供额外的支持，并集成了分子和临床数据。私人和公共界面都将得到设计和支持。这将通过教学互动（研讨会，课程，书面材料和网站），咨询，休假机会和试点资金的结合来实现。此外，我们提出了与技术开发，适应和验证有关的两种壁内活动：在纵向抵抗力训练干预措施中的1,200名志愿者的体积MRI，SNP数据和临床数据的整合，以及QC/SOP的开发和使用代谢标记的蛋白质组分析的QC/SOP和定量分析方法的开发。提出的核心将支持DNA分析（突变研究，SNP发现，SNP基因分型，链接分析，AIM 1）； mRNA分析（微阵列mRNA分析，定量RT-PCR，AIM 2）；和蛋白质组学分析（大分子高吞吐量质谱法，比较蛋白质组学分析，AIM 3）。在拟议的核心中可获得320万美元的最先进设备，以支持NCMRR研究人员进行的所有类型的基因组学和蛋白质组学研究。与拟议的分析核心协同作用的已有NICHD和NCRR资助的核心设施包括MRDDRC遗传学核心，MRDDRC蛋白质组学核心，CRC遗传学核心和CHDRCA遗传学培训核心。