Integrated Molecular Core for Rehabilitation Medicine

描述（由申请人提供）：我们描述了一个拟议的和集成的分子和公共访问核心，以支持NCMRR网络内的康复研究。提议的核心是华盛顿特区国家儿童医学中心遗传医学研究中心的全基因组DNA、mRNA和蛋白质分析专家的一个预先存在的合作小组。拟议的分析和数据库核心的目标是提供与当前医疗康复研究机会特别相关的分子/基因组技术和生物信息学方面的附带专门知识。我们建议在高度并行和/或高通量的DNA， mRNA，蛋白质组学方法方面提供培训，研究资源和合作机会，并提供额外的网络数据库设计和实现支持，并整合分子和临床数据。将设计和支持私有和公共接口。这将通过教学互动（研讨会、课程、书面材料和网站）、咨询、休假机会和试点资金的结合来实现。此外，我们提出了两项与技术开发、适应和验证相关的校内活动：在纵向阻力训练干预中整合1200名志愿者的体积MRI、SNP数据和临床数据，以及开发QC/SOP和使用代谢标记进行蛋白质组学分析的定量分析方法。拟议的核心将支持DNA分析（突变研究，SNP发现，SNP基因分型，连锁分析，Aim 1）；mRNA分析（微阵列mRNA分析，定量RT-PCR， Aim 2）；和蛋白质组学分析（大分子和小分子高通量质谱，比较蛋白质组学分析，Aim 3）。320万美元的最先进设备可用于支持NCMRR研究人员进行的所有类型的基因组学和蛋白质组学研究。现有的NICHD和ncrr资助的核心设施将与拟议的分析核心协同，包括MRDDRC遗传学核心、MRDDRC蛋白质组学核心、CRC遗传学核心和CHDRCA遗传学培训核心。

DESCRIPTION (provided by applicant): We describe a proposed and integrated molecular and public access core in support of rehabilitation research within the NCMRR network. The proposed Core is a pre-existing collaborative group of experts in genomewide DNA, mRNA, and protein analyses at the Research Center for Genetic Medicine, Children's National Medical Center, Washington DC. The goal of the proposed Analytical and Databasing Core is to provide access to collateral expertise in molecular/genomic technologies and bioinformatics that is particularly relevant to current opportunities in medical rehabilitation research. We propose a program of training, research resources, and collaborative opportunities in highly parallel and/or high throughput DNA, mRNA, proteomics methods, with additional support in web database design and implementation, with integration of molecular and clinical data. Both private and public interfaces will be designed and supported. This will be accomplished through a combination of didactic interactions (workshops, courses, written material, and websites), consultations, sabbatical opportunities, and pilot funding. In addition, we propose two intramural activities related to technique development, adaptation, and validation: integration of volumetric MRI, SNP data, and clinical data for 1,200 volunteers in a longitudinal resistance training intervention, and development of QC/SOP and quantitative analysis methods for proteomic profiling using metabolic labeling. The proposed Core will support DNA analyses (mutation studies, SNP discovery, SNP genotyping, linkage analyses, Aim 1); mRNA analyses (microarray mRNA profiling, quantitative RT-PCR, Aim 2); and proteomics analyses (large and small molecule high throughput mass spectrometry, comparative proteomic profiling, Aim 3). $3.2 million of state-of-the-art equipment is available within the proposed core for support of all types of genomics and proteomics studies undertaken by NCMRR investigators. Pre-existing NICHD- and NCRR-funded core facilities that would be synergistic with the proposed Analytical Core include a MRDDRC Genetics Core, MRDDRC Proteomics Core, CRC Genetics Core, and CHDRCA Genetics Training Core.