Integrated Molecular Core for Rehabilitation Medicine

康复医学综合分子核心

• 批准号: 8053664
• 负责人: ERIC P. HOFFMAN
• 金额: $ 1.07万
• 依托单位: CHILDREN'S RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8053664
• 关键词: Adherence; American; Amino Acids; Area; Arts; Bioinformatics; Biological Assay; Child; Chromatography; Clinical; Clinical Data; Clinical Protocols; Clinical Trials; Clinical Trials Network; Collaborations; Computer software; Congresses; Consultations; Core Facility; Cultured Cells; DNA; DNA Fingerprinting; Data; Data Analyses; Data Files; Data Set; Databases; Development; Diagnostic; District of Columbia; Educational Intervention; Educational workshop; Ensure; Equipment; Exercise; Exons; Experimental Designs; Fibroblasts; Funding; Future; Gel; Gene Mutation; Generations; Genetic; Genetic Medicine; Genetic Research; Genome; Genomics; Genotype; Goals; Health Services Research; High Pressure Liquid Chromatography; Human; Image; Individual; Institution; Internet; Intervention Studies; Ions; Knowledge; Label; Laboratories; Licensing; Magnetic Resonance Imaging; Mass Spectrum Analysis; Measurement; Medical center; Medicine; Mental Retardation and Developmental Disabilities Research Centers; Messenger RNA; Metabolic; Methodology; Methods; Mining; Molecular; Molecular Profiling; Mutation; National Institute of Child Health and Human Development; Oligonucleotides; Patients; Phase; Post-Translational Protein Processing; Principal Investigator; Professional counselor; Proteins; Proteomics; Protocols documentation; Public Domains; Publications; Rehabilitation Research; Rehabilitation therapy; Research; Research Infrastructure; Research Personnel; Resource Development; Resources; Reverse Transcriptase Polymerase Chain Reaction; Robot; Robotics; Sampling; Scientist; Screening procedure; Services; Shotguns; Single Nucleotide Polymorphism; Site; Stable Isotope Labeling; Structure; Technology; Testing; Time; Training; Training Programs; Validation; Vision; Work; Writing; base; comparative; computerized data processing; data exchange; database design; design; experience; genetic linkage analysis; genome-wide; human subject; improved; liquid chromatography mass spectrometry; mass spectrometer; member; multidisciplinary; nano; neuromuscular; programs; small molecule; software development; strength training; technique development; therapy development; tumor; volunteer; web interface; web site

DESCRIPTION (provided by applicant): We describe a proposed and integrated molecular and public access core in support of rehabilitation research within the NCMRR network. The proposed Core is a pre-existing collaborative group of experts in genomewide DNA, mRNA, and protein analyses at the Research Center for Genetic Medicine, Children's National Medical Center, Washington DC. The goal of the proposed Analytical and Databasing Core is to provide access to collateral expertise in molecular/genomic technologies and bioinformatics that is particularly relevant to current opportunities in medical rehabilitation research. We propose a program of training, research resources, and collaborative opportunities in highly parallel and/or high throughput DNA, mRNA, proteomics methods, with additional support in web database design and implementation, with integration of molecular and clinical data. Both private and public interfaces will be designed and supported. This will be accomplished through a combination of didactic interactions (workshops, courses, written material, and websites), consultations, sabbatical opportunities, and pilot funding. In addition, we propose two intramural activities related to technique development, adaptation, and validation: integration of volumetric MRI, SNP data, and clinical data for 1,200 volunteers in a longitudinal resistance training intervention, and development of QC/SOP and quantitative analysis methods for proteomic profiling using metabolic labeling. The proposed Core will support DNA analyses (mutation studies, SNP discovery, SNP genotyping, linkage analyses, Aim 1); mRNA analyses (microarray mRNA profiling, quantitative RT-PCR, Aim 2); and proteomics analyses (large and small molecule high throughput mass spectrometry, comparative proteomic profiling, Aim 3). $3.2 million of state-of-the-art equipment is available within the proposed core for support of all types of genomics and proteomics studies undertaken by NCMRR investigators. Pre-existing NICHD- and NCRR-funded core facilities that would be synergistic with the proposed Analytical Core include a MRDDRC Genetics Core, MRDDRC Proteomics Core, CRC Genetics Core, and CHDRCA Genetics Training Core.

描述（由申请人提供）：我们描述了一个提议的集成分子和公共访问核心，以支持 NCMRR 网络内的康复研究。拟议的核心是华盛顿特区儿童国家医疗中心遗传医学研究中心现有的一个由全基因组 DNA、mRNA 和蛋白质分析专家组成的合作小组。拟议的分析和数据库核心的目标是提供分子/基因组技术和生物信息学方面的附带专业知识，这些专业知识与当前医疗康复研究的机会特别相关。我们提出了一个关于高度并行和/或高通量 DNA、mRNA、蛋白质组学方法的培训、研究资源和合作机会的计划，并在网络数据库设计和实施方面提供额外支持，并整合分子和临床数据。私有和公共接口都将被设计和支持。这将通过教学互动（研讨会、课程、书面材料和网站）、咨询、休假机会和试点资金的结合来实现。此外，我们提出了两项​​与技术开发、适应和验证相关的校内活动：在纵向阻力训练干预中整合 1,200 名志愿者的体积 MRI、SNP 数据和临床数据，以及开发 QC/SOP 和使用代谢标记进行蛋白质组分析的定量分析方法。拟议的核心将支持 DNA 分析（突变研究、SNP 发现、SNP 基因分型、连锁分析、目标 1）； mRNA 分析（微阵列 mRNA 分析、定量 RT-PCR、目标 2）；和蛋白质组学分析（大分子和小分子高通量质谱分析、比较蛋白质组学分析、目标 3）。拟议核心内可使用 320 万美元的最先进设备，用于支持 NCMRR 研究人员进行的所有类型的基因组学和蛋白质组学研究。 NICHD 和 NCRR 资助的现有核心设施将与拟议的分析核心协同作用，包括 MRDDRC 遗传学核心、MRDDRC 蛋白质组学核心、CRC 遗传学核心和 CHDRCA 遗传学培训核心。