Pattern formation during Arabidopsis embryogenesis

在植物中，分生组织是一组未分化的干细胞，产生所有器官。BOBBER （BOB）是一个负责正确表达SHOOTMERISTEMLESS （STM）的基因，STM是拟南芥分生组织建立和维持所需的同源结构域基因，BOB突变体在胚胎发生过程中显示STM的空间定位发生改变。对BOB的克隆鉴定出BOB蛋白中的一个NudC结构域。NudC结构域在整个门中都是进化保守的，并在核和细胞迁移、分泌和细胞增殖中发挥作用。研究BOB功能的细胞、分子和遗传机制，以了解该基因在发育模式形成中的作用。其他早期模式突变体将使用一种新的增强子捕获实验来识别。这些研究将产生有用的知识，有助于理解NudC结构域在模式、细胞分裂和分化的调节中的保守作用。NudC结构域已被认为在癌症中发挥作用，这些研究将有助于更深入地了解这些过程。

In plants, the meristem is a group of undifferentiated stem cells that gives rise to all organs. BOBBER (BOB) is a gene responsible for the correct expression of SHOOTMERISTEMLESS (STM), a homeodomain gene required for the establishment and maintenance of Arabidopsis meristems, bob mutants show alterations in the spatial localization of STM during embryogenesis. The cloning of BOB identified a NudC domain in the BOB protein. NudC domains are evolutionarily conserved across phyla and play roles in nuclear and cell migration, secretion, and cell proliferation. Investigation of the cellular, molecular, and genetic mechanisms of BOB function will be undertaken to understand the role of this gene in developmental pattern formation. Other early patterning mutants will be identified using a novel enhancer trapping experiment. These investigations will generate useful knowledge that will contribute to an understanding of the conserved roles of NudC domains in patterning, the regulation of cell division, and differentiation. NudC domains have been implicated to play a role in cancer, and these investigations will contribute to a deeper understanding of these processes.