Pattern formation during Arabidopsis embryogenesis

拟南芥胚胎发生过程中的模式形成

• 批准号: 6844674
• 负责人: NICHOLAS J KAPLINSKY
• 金额: $ 3.44万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6844674
• 关键词: Arabidopsis; cell proliferation; developmental genetics; embryogenesis; immunoprecipitation; mutant; optics; phenotype; plant genetics; plant proteins; postdoctoral investigator; protein protein interaction; protein structure function

In plants, the meristem is a group of undifferentiated stem cells that gives rise to all organs. BOBBER (BOB) is a gene responsible for the correct expression of SHOOTMERISTEMLESS (STM), a homeodomain gene required for the establishment and maintenance of Arabidopsis meristems, bob mutants show alterations in the spatial localization of STM during embryogenesis. The cloning of BOB identified a NudC domain in the BOB protein. NudC domains are evolutionarily conserved across phyla and play roles in nuclear and cell migration, secretion, and cell proliferation. Investigation of the cellular, molecular, and genetic mechanisms of BOB function will be undertaken to understand the role of this gene in developmental pattern formation. Other early patterning mutants will be identified using a novel enhancer trapping experiment. These investigations will generate useful knowledge that will contribute to an understanding of the conserved roles of NudC domains in patterning, the regulation of cell division, and differentiation. NudC domains have been implicated to play a role in cancer, and these investigations will contribute to a deeper understanding of these processes.

在植物中，分生组织是一组未分化的干细胞，产生所有器官。BOBBER（BOB）是一个负责拟南芥分生组织建立和维持所必需的同源结构域基因SHOOTMERISTEMLESS（STM）正确表达的基因，bob突变体在胚胎发生过程中STM的空间定位发生了改变。BOB的克隆鉴定了BOB蛋白中的NudC结构域。NudC结构域在进化上跨门保守，并且在核和细胞迁移、分泌和细胞增殖中发挥作用。BOB功能的细胞，分子和遗传机制的调查将进行了解这个基因在发育模式形成的作用。 其他早期模式突变体将使用一种新的增强子捕获实验进行鉴定。这些研究将产生有用的知识，有助于理解NudC结构域在模式化、细胞分裂和分化调控中的保守作用。NudC结构域被认为在癌症中发挥作用，这些研究将有助于更深入地了解这些过程。