Inner Ear: Sensory Organogenesis and Gene Expression
内耳:感觉器官发生和基因表达
基本信息
- 批准号:6766194
- 负责人:
- 金额:$ 17.53万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-06-01 至 2008-05-31
- 项目状态:已结题
- 来源:
- 关键词:Xenopusauditory pathwayscell differentiationcell growth regulationcell proliferationconfocal scanning microscopydevelopmental geneticsdevelopmental neurobiologyear hair cellelectrophysiologyganglion cellgene expressioninnervationnervous system regenerationneuroanatomyneurogenesisneurogeneticsorgan culturevestibular apparatus
项目摘要
Inner ear disorders of hearing and balance affect over 30 million Americans, many of whom are
children. The goal of this research is to elucidate the cellular and molecular processes that underlie sensory organogenesis during inner ear development. The proposed research will use the acoustico-vestibular systems of Xenopus laevis and Xenopus (Silurana) tropicalis to obtain comparative data about the structure, physiology, and genetic profile of three inner ear endorgans: the sacculus (an acoustico-vestibular organ), the utricle (a vestibular organ) and the amphibian papilla (an acoustic organ). Experiments will focus on mechanosensory hair cells and sensory ganglion cells with the aim of determining how these ceils of the peripheral nervous system acquire their unique phenotype during development. Experiments also aim to discern how mechanosensory hair cells can be restored or regenerated to their normal phenotype after damage or trauma. The specific aims are: (1) to obtain structural and physiological data about the comparative development of the utricle, amphibian papilla, and sacculus, (2) to initiate studies of mechanosensory hair cell development and regeneration using an in vitro culture system, and (3) to determine the function and developmental expression patterns of genes screened from inner ear cDNA libraries. Anatomical and molecular data gathered as part of this research will be prepared for dissemination in the public domain via online databases. Methods used in this research include cell culture, immunohistochemistry, patch clamp, multiphoton and confocal
microscopy, RT-PCR directed cDNA library construction, and yeast two hybrid analysis. A technical objective of this research is to develop methods for vital imaging of ceils and organs of the inner ear.
Experimental results will provide fundamental data about hair cell differentiation and proliferation
during sensory organogenesis, and will increase knowledge of the genetics of the acoustico-vestibular systems. A long term goal of this research is to determine how genes expressed in the inner ear are regulated during development, regeneration, and aging. These investigations aim to produce results with applicability for the development of treatments for hereditary and environmentally-induced disorders of heanng and balance.
听力和平衡的内耳障碍影响了超过3000万美国人,其中许多人是
项目成果
期刊论文数量(0)
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ELBA E SERRANO其他文献
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{{ truncateString('ELBA E SERRANO', 18)}}的其他基金
SENSORY ORGAN FORMATION/ GENE EXPRESSION --INNER EAR DEVELOPMENT
感觉器官形成/基因表达——内耳发育
- 批准号:
6608650 - 财政年份:2002
- 资助金额:
$ 17.53万 - 项目类别:
SENSORY ORGAN FORMATION/ GENE EXPRESSION --INNER EAR DEVELOPMENT
感觉器官形成/基因表达——内耳发育
- 批准号:
6584154 - 财政年份:2002
- 资助金额:
$ 17.53万 - 项目类别:
SENSORY ORGAN FORMATION/ GENE EXPRESSION --INNER EAR DEVELOPMENT
感觉器官形成/基因表达——内耳发育
- 批准号:
6469249 - 财政年份:2001
- 资助金额:
$ 17.53万 - 项目类别:
SENSORY ORGAN FORMATION/ GENE EXPRESSION --INNER EAR DEVELOPMENT
感觉器官形成/基因表达——内耳发育
- 批准号:
6506287 - 财政年份:2001
- 资助金额:
$ 17.53万 - 项目类别:
NEW MEXICO STATE UNIVERSITY MBRS RISE TO EXCELLENCE PROGRAM
新墨西哥州立大学 MBRS 卓越计划
- 批准号:
8114181 - 财政年份:2000
- 资助金额:
$ 17.53万 - 项目类别:
NEW MEXICO STATE UNIVERSITY MBRS RISE TO EXCELLENCE PROGRAM
新墨西哥州立大学 MBRS 卓越计划
- 批准号:
7905152 - 财政年份:2000
- 资助金额:
$ 17.53万 - 项目类别:
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