QUANTUM DOT TOXICITY IN HUMAN CELLS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The potential health risks of nanomaterials are not well understood and are a subject of increasing concern. In this study we implement flow cytometry to assess the viability of human embryonic kidney cells that have been exposed to quantum dot (QD) nanoparticles. Flow cytometric analysis has confirmed the QD label was present in cells up to 48 hours after exposure. Results also indicate that cell viabilityt as measured by cell cycle profile (Hoechst 33342), mitochondrial function (Mitotraker dye) and cell morphometry were similar between control and experimental (QD) cell cultures.