QUANTUM DOT TOXICITY IN HUMAN CELLS

人体细胞中的量子点毒性

• 批准号: 7724261
• 负责人: ELBA E SERRANO
• 金额: $ 1.61万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724261
• 关键词: Cell Cycle; Cells; Computer Retrieval of Information on Scientific Projects Database; Cultured Cells; Dyes; Embryo; Flow Cytometry; Funding; Grant; HOE 33342; Health; Hour; Human; Institution; Label; Measures; Mitochondria; Quantum Dots; Research; Research Personnel; Resources; Risk; Source; Toxic effect; United States National Institutes of Health; kidney cell; morphometry; nanomaterials; nanoparticle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The potential health risks of nanomaterials are not well understood and are a subject of increasing concern. In this study we implement flow cytometry to assess the viability of human embryonic kidney cells that have been exposed to quantum dot (QD) nanoparticles. Flow cytometric analysis has confirmed the QD label was present in cells up to 48 hours after exposure. Results also indicate that cell viabilityt as measured by cell cycle profile (Hoechst 33342), mitochondrial function (Mitotraker dye) and cell morphometry were similar between control and experimental (QD) cell cultures.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 纳米材料的潜在健康风险尚未得到充分了解，并且成为日益受到关注的主题。 在这项研究中，我们采用流式细胞术来评估暴露于量子点 (QD) 纳米颗粒的人胚胎肾细胞的活力。 流式细胞术分析证实 QD 标签在暴露后 48 小时内仍存在于细胞中。 结果还表明，通过细胞周期概况 (Hoechst 33342)、线粒体功能（Mitotraker 染料）和细胞形态测定测量的细胞活力在对照和实验 (QD) 细胞培养物之间相似。