The BD FACSAria, a new flow cytometer cell sorter

描述(由申请者提供)：申请资金以获得Becton Dickinson FACSAria TM细胞分类器，以进行各种生物医学相关研究。BD FACSAria是一种新开发的FACS分选机，在其优越的应用性能和较低的维护要求方面超过了以前的型号FACSVantage。由于亚利桑那州生物设计研究所(AZ Bio-Design)的成立和亚利桑那州立大学最近扩大了生物医学研究活动，该仪器的问世恰逢其时。已经形成了一个由8名国家卫生研究院受赠人组成的主要用户小组，其中7人是这项赠款申请的调查人员(见下文)。该小组的研究活动包括：确定V(D)J重组事件中潜在的染色体易位；开发针对乙肝病毒和天花的疫苗；测定热休克蛋白20在平滑肌松弛中的功能活性；确定抗癌药物的分子靶点；构建和筛选用于治疗神经退行性疾病的重组噬菌体展示抗体；评估心理疗法治疗类风湿性关节炎的效果；以及表征细菌对抗生素的耐药性。此外，亚利桑那州立大学的其他研究人员将使用这种设备来协助他们在蛋白质传递、光合作用和免疫调节方面的研究。所要求的仪器将极大地提高国家卫生研究院受赠者和亚利桑那州立大学其他研究人员开展研究活动和探索新的研究方向的能力，否则是不可能的。该仪器将是AZ生物设计公司核心设施的一部分，并将由AZ生物设计公司的一个内部咨询委员会管理。费用将由NIH用户、AZBioDesign和该大学分担。为了最大限度地利用这一核心设施，我们将继续努力扩大用户群体，包括校园外的研究人员。

DESCRIPTION (provided by applicant): Funds are requested to obtain a Becton Dickinson FACSAria TM Cell Sorter to conduct a variety of biomedical related studies. The BD FACSAria is a newly developed FACS sorter, surpassing the earlier model, FACSVantage, in its superior application performance and low maintenance requirement. The availability of this instrument comes at a perfect time when great demand is presented as a result of establishment of Arizona BioDesign Institute (AZ Bio-Design) and recent expansion of ASU biomedical research activities. A major user group consisting of eight NIH grantees has been formed, of whom seven are the investigators on this grant application (see below). The research activities of this group involve identification of potential chromosomal translocation during attempted V(D)J recombination events; development of vaccines against HBV and smallpox; determination of functional activity of Heat-Shock Protein 20 in smooth muscle relaxation; identification of molecular targets of anti-cancer drugs; construction and screening recombinant phage-display antibodies for treating neurodegenerative diseases; assessment of psychotherapy in treating Rheumatoid Arthritis and characterization of outer membrane proteins in bacterial antibiotic resistance. In addition, other investigators at ASU will use this equipment to assist their studies in protein delivery, photosynthesis and immune modulation. The requested instrument will greatly enhance the ability of NIH grantees and other investigators at ASU to perform their research activities and to explore new research directions that would not be possible otherwise. This instrument will be part of a core facility of AZ Bio-Design, and will be administered by an internal advisory committee at AZ Bio-Design. Cost will be shared among NIH users, AZBioDesign and the university. To maximize the usage of this core facility, we will continue our effort to expand the user group, including researchers outside the campus.