The BD FACSAria, a new flow cytometer cell sorter

描述（由申请人提供）：申请资金以获得Becton Dickinson FACSAria TM细胞分选仪，以进行各种生物医学相关研究。BD FACSAria是一款新开发的流式细胞仪分选机，在上级应用性能和低维护要求方面超越了早期型号FACSVantage。该仪器的可用性是在一个完美的时间，当巨大的需求是由于亚利桑那州生物设计研究所（AZ生物设计）的建立和亚利桑那州立大学生物医学研究活动的最近扩展。一个主要的用户组由八个NIH赠款人组成，其中七个是这项赠款申请的研究人员（见下文）。该研究组的研究活动包括鉴定在尝试的V（D）J重组事件中潜在的染色体易位;开发针对HBV和天花的疫苗;测定热休克蛋白20在平滑肌松弛中的功能活性;鉴定抗癌药物的分子靶点;构建和筛选用于治疗神经变性疾病的重组噬菌体展示抗体;风湿性关节炎心理治疗的评估和细菌抗生素耐药性外膜蛋白的表征。此外，亚利桑那州立大学的其他研究人员将使用该设备来协助他们在蛋白质递送、光合作用和免疫调节方面的研究。所要求的工具将大大提高NIH受资助者和亚利桑那州立大学其他研究人员开展研究活动的能力，并探索新的研究方向，否则将不可能。该仪器将成为AZ Bio-Design核心设施的一部分，并将由AZ Bio-Design的内部咨询委员会管理。费用将由NIH用户、AZBioDesign和大学分担。为了最大限度地利用这一核心设施，我们将继续努力扩大用户群，包括校园外的研究人员。

DESCRIPTION (provided by applicant): Funds are requested to obtain a Becton Dickinson FACSAria TM Cell Sorter to conduct a variety of biomedical related studies. The BD FACSAria is a newly developed FACS sorter, surpassing the earlier model, FACSVantage, in its superior application performance and low maintenance requirement. The availability of this instrument comes at a perfect time when great demand is presented as a result of establishment of Arizona BioDesign Institute (AZ Bio-Design) and recent expansion of ASU biomedical research activities. A major user group consisting of eight NIH grantees has been formed, of whom seven are the investigators on this grant application (see below). The research activities of this group involve identification of potential chromosomal translocation during attempted V(D)J recombination events; development of vaccines against HBV and smallpox; determination of functional activity of Heat-Shock Protein 20 in smooth muscle relaxation; identification of molecular targets of anti-cancer drugs; construction and screening recombinant phage-display antibodies for treating neurodegenerative diseases; assessment of psychotherapy in treating Rheumatoid Arthritis and characterization of outer membrane proteins in bacterial antibiotic resistance. In addition, other investigators at ASU will use this equipment to assist their studies in protein delivery, photosynthesis and immune modulation. The requested instrument will greatly enhance the ability of NIH grantees and other investigators at ASU to perform their research activities and to explore new research directions that would not be possible otherwise. This instrument will be part of a core facility of AZ Bio-Design, and will be administered by an internal advisory committee at AZ Bio-Design. Cost will be shared among NIH users, AZBioDesign and the university. To maximize the usage of this core facility, we will continue our effort to expand the user group, including researchers outside the campus.