The BD FACSAria, a new flow cytometer cell sorter

描述（由申请人提供）：申请资金用于购买Becton Dickinson FACSAria TM细胞分选仪进行各种生物医学相关研究。BD FACSAria是新开发的FACS分选机，超越了早期型号facsadvantage，具有优越的应用性能和低维护要求。由于亚利桑那州生物设计研究所（AZ Bio-Design）的建立和最近亚利桑那州立大学生物医学研究活动的扩大，对该仪器的需求很大，因此该仪器的可用性恰逢其时。一个由8名NIH受助者组成的主要用户组已经成立，其中7人是这项拨款申请的调查人员（见下文）。该组的研究活动包括在尝试V(D)J重组事件中识别潜在的染色体易位；研制乙型肝炎病毒和天花疫苗；热休克蛋白20在平滑肌松弛中的功能活性测定；抗癌药物分子靶点的鉴定；治疗神经退行性疾病重组噬菌体展示抗体的构建与筛选类风湿关节炎的心理治疗评估及细菌抗生素耐药性的外膜蛋白特征。此外，亚利桑那州立大学的其他研究人员将使用该设备协助他们在蛋白质传递、光合作用和免疫调节方面的研究。所要求的仪器将大大提高美国国立卫生研究院的受助者和其他研究者在亚利桑那州立大学进行他们的研究活动和探索新的研究方向的能力，否则将不可能。该仪器将成为AZ Bio-Design核心设施的一部分，并将由AZ Bio-Design的内部咨询委员会管理。费用将由NIH用户、AZBioDesign和该大学共同承担。为了最大限度地利用这一核心设施，我们将继续努力扩大用户群，包括校园外的研究人员。

DESCRIPTION (provided by applicant): Funds are requested to obtain a Becton Dickinson FACSAria TM Cell Sorter to conduct a variety of biomedical related studies. The BD FACSAria is a newly developed FACS sorter, surpassing the earlier model, FACSVantage, in its superior application performance and low maintenance requirement. The availability of this instrument comes at a perfect time when great demand is presented as a result of establishment of Arizona BioDesign Institute (AZ Bio-Design) and recent expansion of ASU biomedical research activities. A major user group consisting of eight NIH grantees has been formed, of whom seven are the investigators on this grant application (see below). The research activities of this group involve identification of potential chromosomal translocation during attempted V(D)J recombination events; development of vaccines against HBV and smallpox; determination of functional activity of Heat-Shock Protein 20 in smooth muscle relaxation; identification of molecular targets of anti-cancer drugs; construction and screening recombinant phage-display antibodies for treating neurodegenerative diseases; assessment of psychotherapy in treating Rheumatoid Arthritis and characterization of outer membrane proteins in bacterial antibiotic resistance. In addition, other investigators at ASU will use this equipment to assist their studies in protein delivery, photosynthesis and immune modulation. The requested instrument will greatly enhance the ability of NIH grantees and other investigators at ASU to perform their research activities and to explore new research directions that would not be possible otherwise. This instrument will be part of a core facility of AZ Bio-Design, and will be administered by an internal advisory committee at AZ Bio-Design. Cost will be shared among NIH users, AZBioDesign and the university. To maximize the usage of this core facility, we will continue our effort to expand the user group, including researchers outside the campus.