FLOW CYTOMETRY AND CELL SORTING

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 流式细胞术和细胞分选的核心设施不仅为OMRF，而且为健康科学校园和整个俄克拉荷马州的其他人提供了技术支持。其他机构有分析仪，其中两个能够对细胞进行分类。然而，OMRF的设施是独一无二的最先进的，经常帮助其他人解决技术问题。该COBRE的调查人员不仅可以优先使用仪器，还可以优先接受培训。这很重要，因为我们已经了解到，科学家得到最大限度的 当他们至少能够分析自己的数据时，他们的实验会产生什么结果。有些研究员甚至学习如何操作FACSAria，但这是在设施技术人员的密切监督下进行的。培训更进一步，鼓励经验丰富的用户与新用户分享多色染色和珠子分离技术。 Lijun Xia、Courtney Griffin、Jana Barlic、Hong Chen和Tim Griffin博士的项目都需要多参数流式细胞术和/或细胞分选。有必要从肝脏中提取稀有细胞， 血液脂肪组织皮肤和骨髓这些包括淋巴内皮细胞、血液内皮细胞、单核细胞、巨噬细胞和其它白细胞。许多OMRF科学家已经有经验， 计数和分选极其罕见的群体，如干细胞。此外，他们使用该设施来确定移植小鼠中嵌合体的程度以及表达或敲低构建体的功效。因此，所提议或可能采取的一切行动都在核心基金的能力范围之内。作为指导职能的一部分，将向COBRE调查人员建议新的和更翔实的实验方案。最后，核心基金将通过向他们提供必要的最先进的支持，帮助招募更多有才华的科学家。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Core Facility for Flow Cytometry and Cell Sorting provides enabling technology not only for OMRF, but for others on the Health Sciences Campus and throughout the state of Oklahoma. Other institutions have analyzers, and two are able to sort cells. However, OMRF's facility is uniquely state of the art and has often helped others with technical issues. Investigators in this COBRE will have priority access not only to the instruments, but also to training. This is important because we have learned that scientists get maximum yield from their experiments when they are at least able to analyze their own data. Some fellows even learn how to operate the FACSAria, but this is done under close supervision by Facility technicians. Training goes even further by encouraging experienced users to share multi-color staining and bead separation technology with new users. The projects of Drs. Lijun Xia, Courtney Griffin, Jana Barlic, Hong Chen and Tim Griffin all require multiparameter flow cytometry and/or cell sorting. It will be necessary to manipulate rare cell types from liver, blood, adipose tissues, skin and bone marrow. These include lymphatic endothelial cells, blood endothelial cells, monocytes, macrophages and other leukocytes. Many OMRF scientists are already experienced with enumeration and sorting of extremely rare populations such as stem cells. Additionally, they use the facility to determine the extent of chimerism in transplanted mice and the efficacy of expression or knockdown constructs. Thus, everything that has been proposed or is likely to be done is within the capabilities of the Core Facility. As part of the mentoring function, new and more informative experimental protocols will be suggested to COBRE investigators. Finally, the Core Facility will help to recruit additional talented scientists by providing them with essential, state-of-the-art support.