FLOW CYTOMETRY AND CELL SORTING
FLOW CYTOMETRY AND CELL SORTING
批准号:
8364982
负责人:
Linda F Thompson
金额:
$12.03万
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-05-01 至 2012-04-30
关键词:
Adipose tissueBiologyBloodBlood VesselsBone MarrowCell SeparationCenters of Research ExcellenceChimerismColorCore FacilityDataEndothelial CellsFlow CytometryFundingGrantHealth SciencesInstitutionInterdisciplinary StudyLearningLeukocytesLiverLymphatic Endothelial CellsMentorsMusNational Center for Research ResourcesOklahomaPopulationPrincipal InvestigatorProtocols documentationRecruitment ActivityResearchResearch InfrastructureResearch PersonnelResourcesScientistSkinSorting - Cell MovementSourceStaining methodStainsStem cellsSupervisionTechnologyTrainingTransplantationUnited States National Institutes of Healthcell typecostexperienceinstrumentmacrophagemonocyteresearch study
中文摘要
这个子项目是利用这些资源的众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
The Core Facility for Flow Cytometry and Cell Sorting provides enabling technology not only for OMRF, but for others on the Health Sciences Campus and throughout the state of Oklahoma. Other institutions have analyzers, and two are able to sort cells. However, OMRF's facility is uniquely state of the art and has often helped others with technical issues. Investigators in this COBRE will have priority access not only to the instruments, but also to training. This is important because we have learned that scientists get maximum
yield from their experiments when they are at least able to analyze their own data. Some fellows even learn how to operate the FACSAria, but this is done under close supervision by Facility technicians. Training goes even further by encouraging experienced users to share multi-color staining and bead separation technology with new users.
The projects of Drs. Lijun Xia, Courtney Griffin, Jana Barlic, Hong Chen and Tim Griffin all require multiparameter flow cytometry and/or cell sorting. It will be necessary to manipulate rare cell types from liver,
blood, adipose tissues, skin and bone marrow. These include lymphatic endothelial cells, blood endothelial cells, monocytes, macrophages and other leukocytes. Many OMRF scientists are already experienced with
enumeration and sorting of extremely rare populations such as stem cells. Additionally, they use the facility to determine the extent of chimerism in transplanted mice and the efficacy of expression or knockdown constructs. Thus, everything that has been proposed or is likely to be done is within the capabilities of the Core Facility. As part of the mentoring function, new and more informative experimental protocols will be suggested to COBRE investigators. Finally, the Core Facility will help to recruit additional talented scientists by providing them with essential, state-of-the-art support.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Administrative Core
-
批准号:10629615
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项目类别:
-
资助金额:$18.78万
-
财政年份:2023
-
负责人:Linda F Thompson
-
依托单位:
Expanding Excellence in Developmental Biology in Oklahoma
-
批准号:10629614
-
项目类别:
-
资助金额:$127.88万
-
财政年份:2023
-
负责人:Linda F Thompson
-
依托单位:
Administrative Core
-
批准号:10474289
-
项目类别:
-
资助金额:$74.99万
-
财政年份:2013
-
负责人:Linda F Thompson
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依托单位:
ConProject-003
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批准号:10917526
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项目类别:
-
资助金额:$64.07万
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财政年份:2013
-
负责人:Linda F Thompson
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依托单位:
Expanding Excellence in Developmental Biology in Oklahoma Supplement: 3D Human Lung Tissue Model to Dissect Cellular Responses upon SARS-CoV-2 Infection
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批准号:10853526
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项目类别:
-
资助金额:$64.07万
-
财政年份:2013
-
负责人:Linda F Thompson
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依托单位:
Expanding Excellence in Developmental Biology in Oklahoma
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批准号:9234544
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项目类别:
-
资助金额:$252.0万
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财政年份:2013
-
负责人:Linda F Thompson
-
依托单位:
Expanding Excellence in Developmental Biology in Oklahoma
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批准号:8432235
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项目类别:
-
资助金额:$282.0万
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财政年份:2013
-
负责人:Linda F Thompson
-
依托单位:
Expanding Excellence in Developmental Biology in Oklahoma
-
批准号:10225569
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项目类别:
-
资助金额:$256.36万
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财政年份:2013
-
负责人:Linda F Thompson
-
依托单位:
Expanding Excellence in Developmental Biology in Oklahoma
-
批准号:8625776
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项目类别:
-
资助金额:$252.0万
-
财政年份:2013
-
负责人:Linda F Thompson
-
依托单位:
Administrative Core
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批准号:10225570
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项目类别:
-
资助金额:$61.92万
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财政年份:2013
-
负责人:Linda F Thompson
-
依托单位:
Expanding Excellence in Developmental Biology in Oklahoma
-
批准号:10474288
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项目类别:
-
资助金额:$256.36万
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财政年份:2013
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负责人:Linda F Thompson
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依托单位:
CORE B: PILOT CORES
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批准号:8364937
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项目类别:
-
资助金额:$20.4万
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财政年份:2011
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负责人:Linda F Thompson
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依托单位:
HUMAN ANTIBODY CORE
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批准号:7959382
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项目类别:
-
资助金额:$13.62万
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财政年份:2009
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负责人:Linda F Thompson
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依托单位:
Flow Cytometry Core
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批准号:10686295
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项目类别:
-
资助金额:$58.87万
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财政年份:2004
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负责人:Linda F Thompson
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依托单位:
Flow Cytometry Core
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批准号:10237854
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项目类别:
-
资助金额:$8.39万
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财政年份:2004
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负责人:Linda F Thompson
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依托单位:
Flow Cytometry Core
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批准号:10469991
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项目类别:
-
资助金额:$59.17万
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财政年份:2004
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负责人:Linda F Thompson
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依托单位:
Complement and coagulation in B. anthracis peptidoglycan-induced sepsis
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批准号:10469994
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项目类别:
-
资助金额:$45.84万
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财政年份:2004
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负责人:Linda F Thompson
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依托单位:
Flow Cytometry Core
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批准号:9927971
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项目类别:
-
资助金额:$8.42万
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财政年份:2004
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负责人:Linda F Thompson
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依托单位:
Early Events in Human T Cell Development
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批准号:6340725
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项目类别:
-
资助金额:$15.5万
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财政年份:2000
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负责人:Linda F Thompson
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依托单位:
MOLECULAR ASPECTS OF HUMAN LYMPHOPOIESIS
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批准号:6446848
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项目类别:
-
资助金额:$81.88万
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财政年份:1999
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负责人:Linda F Thompson
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依托单位:
国内基金
海外基金
Journal of Integrative Plant Biology
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批准号:31024801
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项目类别:专项基金项目
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资助金额:24.0万元
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批准年份:2010
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负责人:贺萍
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依托单位: