FLOW CYTOMETRY AND CELL SORTING

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 用于流式细胞术和细胞分选的核心设备不仅为OMRF提供支持技术，而且为健康科学校园和整个俄克拉何马州的其他人提供支持技术。其他机构拥有分析仪，其中两家能够对细胞进行分类。然而，OMRF的设施是独一无二的最先进的，经常帮助其他人解决技术问题。科布雷的调查人员不仅可以优先获得仪器，还可以接受培训。这一点很重要，因为我们已经了解到，科学家可以最大限度地 当他们至少能够分析自己的数据时，就会从他们的实验中获益。一些研究员甚至学习如何操作FACSAria，但这是在设施技术人员的密切监督下完成的。培训更进一步，鼓励有经验的用户与新用户分享多色染色和珠粒分离技术。 夏丽君、柯特妮·格里芬、贾娜·巴里克、陈红和蒂姆·格里芬博士的项目都需要多参数流式细胞仪和/或细胞分选。有必要操控来自肝脏稀有细胞类型， 血液、脂肪组织、皮肤和骨髓。这些细胞包括淋巴管内皮细胞、血液内皮细胞、单核细胞、巨噬细胞和其他白细胞。许多OMRF科学家已经有了经验 对干细胞等极其稀有的种群进行计数和分类。此外，他们还使用该设备来确定移植小鼠中嵌合体的程度以及表达或敲除结构的有效性。因此，提议或可能做的一切都在核心机制的能力范围内。作为指导功能的一部分，将向Cobre研究人员建议新的和更有信息量的实验方案。最后，核心设施将帮助招募更多有才华的科学家，为他们提供必要的、最先进的支持。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Core Facility for Flow Cytometry and Cell Sorting provides enabling technology not only for OMRF, but for others on the Health Sciences Campus and throughout the state of Oklahoma. Other institutions have analyzers, and two are able to sort cells. However, OMRF's facility is uniquely state of the art and has often helped others with technical issues. Investigators in this COBRE will have priority access not only to the instruments, but also to training. This is important because we have learned that scientists get maximum yield from their experiments when they are at least able to analyze their own data. Some fellows even learn how to operate the FACSAria, but this is done under close supervision by Facility technicians. Training goes even further by encouraging experienced users to share multi-color staining and bead separation technology with new users. The projects of Drs. Lijun Xia, Courtney Griffin, Jana Barlic, Hong Chen and Tim Griffin all require multiparameter flow cytometry and/or cell sorting. It will be necessary to manipulate rare cell types from liver, blood, adipose tissues, skin and bone marrow. These include lymphatic endothelial cells, blood endothelial cells, monocytes, macrophages and other leukocytes. Many OMRF scientists are already experienced with enumeration and sorting of extremely rare populations such as stem cells. Additionally, they use the facility to determine the extent of chimerism in transplanted mice and the efficacy of expression or knockdown constructs. Thus, everything that has been proposed or is likely to be done is within the capabilities of the Core Facility. As part of the mentoring function, new and more informative experimental protocols will be suggested to COBRE investigators. Finally, the Core Facility will help to recruit additional talented scientists by providing them with essential, state-of-the-art support.