Micromass QTof Hybrid Mass Spectrometer

描述（由申请人提供）：向NCRR申请共享仪器拨款，用于Micromass混合四极杆飞行时间（Q ToF）质谱仪。该仪器将被安置在整合基因组学中心（CIG），这是伯克利校园的一个新研究单位。该仪器将用于蛋白质鉴定后的一或二维凝胶电泳，使用MS-MS测序的蛋白水解肽。该仪器支持的项目将涉及人类和四膜虫端粒酶、果蝇、Ciona和Artmia转录因子、果蝇P元件转座酶和DNA修复蛋白、果蝇RNA结合/ RNA剪接因子和爪蟾胚胎转录和信号转导因子。该仪器还将用于分析转录因子、DNA重组酶、端粒酶和RNA剪接因子的蛋白质磷酸化，使用纳米lc MS-MS和前体离子扫描在正或负模式下。这种使用纳米电喷雾电离和混合四极杆飞行时间质量分析仪的仪器在加州大学伯克利分校是不可用的。

DESCRIPTION (provided by applicant): A request is made for a shared instrumentation grant to NCRR for a Micromass hybrid quadrupole time of flight (Q ToF) mass spectrometer. The instrument will be housed in the Center for Integrated Genomics (CIG), a new research unit on the Berkeley campus. The instrument will be used for protein identification following one- or two-dimensional gel electrophoresis, using MS-MS sequencing of proteolytic peptides. Projects supported by this instrument will involve human and Tetrahymena telomerase, Drosophila, Ciona and Artmia transcription factors, Drosophila P element transposase and DNA repair proteins, Drosophila RNA binding / RNA splicing factors and Xenopus embryonic transcription and signal transduction factors. The instrument will also be used to analyze protein phosphorylation of transcription factors, DNA recombination enzymes, telomerase and RNA splicing factors using nano-LC MS-MS and precursor ion scanning in the positive or negative mode. An instrument of this type, using nano-elctrospray ionization in conjunction with a hybrid quadrupole-time-of-flight mass analyzer is not available on the U.C., Berkeley campus.