Cytomation MoFlo cytometer and high-speed cell sorter

Cytomation MoFlo 细胞仪和高速细胞分选仪

• 批准号: 6581548
• 负责人: Mary B Tompkins
• 金额: $ 45.23万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6581548
• 关键词: biomedical equipment purchase; cell biology; flow cytometry

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a Cytomotion MoFlo high speed, high purity flow cytometric cell sorter to support the research of nine (9) Biomedical research faculty with a total of 11 NIH supported projects at North Carolina State University. The instrument will be housed in the Flow Cytometry Core Facility at NCSU. Some of the projects focus on small subsets of T lymphocytes that regulate resistance or immunodeficiency in the FIV-model for HIV-induced AIDS. Two projects examine bronchial epithelial cell populations and their functions. Another project includes utilizing knock-out mice to study the factors of cells involved in chronic inflammatory bowel disease; utilizing transfected mutated transcription factors to analyze expression of specific endo cell-cycle genes regulating susceptibility to retinoblastoma; and gene targeting and animal cloning technology. In all cases, MoFIo technology is required to separate cell subsets based on either surface markers or cell cycle to analyze specific proteins and/or cell function. The PIs currently have available a FACSCaliber for cell sorting, but its sorting speed (500 cells/sec) to too slow. The MoFIo sorts at a rate up to 70,000 cells/sec, and has the capability of simultaneous sorting of up to 4 cell subsets from a mixed population. There is a MoFIo at the University of North Carolina-Chapel Hill and two FACSVantage sortesr at Duke University, both 30 miles from NCSU. The difficulty in scheduling these instruments and long commuting time (minimum of 45 minutes one way at best) makes it impractical to use this technology on a routine basis. The University will provide support for the MoFIo operator and costs to place the instrument in operational order. Users fees established by the MoFlo oversight committee will cover subsequent service contracts. Client use of the MoFIo will be determined by the oversight committee under current guidelines for the Flow Cytometry Facility. The PIs on this proposal will be given priority; however, other faculty will be encouraged to use the technology as the need arises.

描述（由申请人提供）：本提案要求提供资金，用于购买Cytomotion MoFlo高速、高纯度流式细胞仪，以支持北卡罗来纳州州立大学的九（9）个生物医学研究学院的研究，共11个NIH支持的项目。仪器将存放在NCSU的流式细胞术核心设施中。其中一些项目集中于调节HIV诱导的AIDS的FIV模型中的抵抗力或免疫缺陷的T淋巴细胞的小亚群。两个项目研究支气管上皮细胞群及其功能。另一个项目包括利用基因敲除小鼠研究慢性炎症性肠病相关细胞的因子;利用转染的突变转录因子分析调节视网膜母细胞瘤易感性的特定内细胞周期基因的表达;以及基因靶向和动物克隆技术。在所有情况下，都需要MoFIo技术根据表面标志物或细胞周期分离细胞亚群，以分析特定蛋白质和/或细胞功能。PI目前有FACSCaliber用于细胞分选，但其分选速度（500个细胞/秒）太慢。MoFIo的分选速度高达70，000个细胞/秒，并且能够从混合群体中同时分选多达4个细胞亚群。北卡罗来纳大学教堂山分校有一个MoFIo，杜克大学有两个FACSVantage分拣机，两个都离NCSU 30英里。安排这些仪器的困难和长的通勤时间（单程最少45分钟）使得在常规基础上使用该技术不切实际。该大学将为金融情报部的操作员提供支持，并支付使仪器投入运行的费用。交通和物流部监督委员会确定的用户费将用于支付随后的服务合同。客户对MoFIo的使用将由监督委员会根据流式细胞仪设施的现行指导原则决定。该提案的PI将被优先考虑;然而，其他教师将被鼓励在需要时使用该技术。