BD FACS Vantage II Cell Sorter

描述（由申请人提供）：Sidney Kimmel癌症中心是一个非营利性研究机构，成立于1991年，由USPH资助，研究项目包括癌症基因组学，血管生成和血管生物学，造血和免疫学以及分子和细胞生物学。该中心发展迅速，目前拥有21名主要研究人员和130名支持人员，每年获得NIH 1200万美元的支持。目前，悉尼坎摩尔癌症中心的员工占地9万平方英尺。占地面积38,000平方英尺。正在兴建的大厦，预计于2003年7月投入使用。流式细胞仪核心设备正在扩建中。目前，该设施包括一个Becton Dickinson FACS Calibur仪器，配备四个荧光参数检测和五个计算机工作站，分布在整个中心，具有Cell Quest数据分析软件，并联网到具有24gb存储容量的NT Windows服务器。目前，每个月都有针对新员工的培训课程，关于流式细胞术各种用途的系列研讨会正在开发中。获得B.D. FACS Vantage SE II型细胞仪将为该中心的用户以及圣地亚哥地区更大的研究社区提供最先进的荧光激活细胞分选能力。Sidney Kimmel癌症中心已经确定了8个主要用户和6个次要用户，他们的研究项目要么需要，要么将通过获得这项技术而显着增强。所有主要用户和大多数次要用户都有流式细胞术的经验，许多人经常使用邻近研究机构的细胞分选设备。大多数用户需要收集大量具有明确表型的纯化细胞，用于各种体外和体内试验，其他用户需要筛选和选择成功转染的细胞系、细菌，或从大量样本中纯化非常罕见的细胞，用于基因表达分析。FACS Vantage SE II仪器将非常适合这些需求，它具有多参数（多达8个荧光和2个光散射参数）检测的能力，4路分选允许同时收集四个亚组，自动分选到多孔托盘中，分选速度为25,000次/秒，保留了出色的活力，功能和纯度。

DESCRIPTION (provided by applicant): The Sidney Kimmel Cancer Center is a nonprofit research institute founded in 1991 with USPH grant-supported research programs in cancer genomics, angiogenesis and vascular biology, hematopoiesis and immunology and molecular and cell biology. The Center has grown rapidly to our current roster of 21 principal investigators and 130 support staff with $12 million/year in NIH support. Presently employees of the Sidney Kimmel Cancer Center occupy a 90,000 sq. ft. building with an adjacent 38,000 sq. ft. building under construction, to be occupied in July 2003. A flow cytometry core facility is under expansion. Presently the facility consists of a Becton Dickinson FACS Calibur instrument equipped for four fluorescence parameter detection and five computer workstations located throughout the Center having Cell Quest data analysis software and networked to a NT Windows server having a 24 gigabyte storage capacity. Currently there are monthly training classes for new staff and a seminar series on the various uses of flow cytometry is under development. The acquisition of a B.D. FACS Vantage SE II cytometer would provide state-of-the art fluorescence-activated cell sorting capability, both to users at the Center but also to the larger research community in the San Diego area. Eight major and six minor users have been identified at the Sidney Kimmel Cancer Center whose research programs either require or would be significantly enhanced by access to this technology. All of the major users and most of the minor users have experience with flow cytometry and many routinely utilize cell sorting facilities at neighboring research institutes. The majority of the users have need to collect large numbers of purified cells with a defined phenotype for use in a variety of in vitro and in vivo assays, others require the screening and selection of successfully transfected cell lines, bacteria, or the purification of very rare cells from large samples for gene expression analyses. The FACS Vantage SE II instrument would be ideally suited to these needs with its capacity for multi-parameter (up to 8 fluorescence and 2 light scatter parameters) detection, 4-way sorting allowing the simultaneous collection of four subsets, automated sorting into multi-well trays and sorting speeds of 25,000 events/second with retention of excellent viability, function and purity.