BD FACS Vantage II Cell Sorter

BD FACS Vantage II 细胞分选仪

• 批准号: 6580994
• 负责人: Phyllis-Jean Linton
• 金额: $ 50万
• 依托单位: SIDNEY KIMMEL CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580994
• 关键词: biomedical equipment purchase; cell sorting; flow cytometry

DESCRIPTION (provided by applicant): The Sidney Kimmel Cancer Center is a nonprofit research institute founded in 1991 with USPH grant-supported research programs in cancer genomics, angiogenesis and vascular biology, hematopoiesis and immunology and molecular and cell biology. The Center has grown rapidly to our current roster of 21 principal investigators and 130 support staff with $12 million/year in NIH support. Presently employees of the Sidney Kimmel Cancer Center occupy a 90,000 sq. ft. building with an adjacent 38,000 sq. ft. building under construction, to be occupied in July 2003. A flow cytometry core facility is under expansion. Presently the facility consists of a Becton Dickinson FACS Calibur instrument equipped for four fluorescence parameter detection and five computer workstations located throughout the Center having Cell Quest data analysis software and networked to a NT Windows server having a 24 gigabyte storage capacity. Currently there are monthly training classes for new staff and a seminar series on the various uses of flow cytometry is under development. The acquisition of a B.D. FACS Vantage SE II cytometer would provide state-of-the art fluorescence-activated cell sorting capability, both to users at the Center but also to the larger research community in the San Diego area. Eight major and six minor users have been identified at the Sidney Kimmel Cancer Center whose research programs either require or would be significantly enhanced by access to this technology. All of the major users and most of the minor users have experience with flow cytometry and many routinely utilize cell sorting facilities at neighboring research institutes. The majority of the users have need to collect large numbers of purified cells with a defined phenotype for use in a variety of in vitro and in vivo assays, others require the screening and selection of successfully transfected cell lines, bacteria, or the purification of very rare cells from large samples for gene expression analyses. The FACS Vantage SE II instrument would be ideally suited to these needs with its capacity for multi-parameter (up to 8 fluorescence and 2 light scatter parameters) detection, 4-way sorting allowing the simultaneous collection of four subsets, automated sorting into multi-well trays and sorting speeds of 25,000 events/second with retention of excellent viability, function and purity.

Sidney Kimmel癌症中心是一家非营利性研究机构，成立于1991年，由USPH资助，在癌症基因组学，血管生成和血管生物学，造血和免疫学以及分子和细胞生物学方面开展研究计划。该中心已迅速发展到目前的21名主要研究人员和130名支持人员，每年获得NIH的1200万美元支持。目前，西德尼·金梅尔癌症中心的员工占地90，000平方米。英尺建筑面积38,000平方米，英尺大楼正在建设中，将于2003年7月启用。流式细胞术核心设施正在扩建。目前，该设施包括一台配备有四个荧光参数检测的Becton Dickinson FACS Calibur仪器和五个位于整个中心的计算机工作站，这些工作站具有Cell Quest数据分析软件，并与具有24千兆字节存储容量的NT Windows服务器联网。目前，每月为新工作人员举办培训班，并正在举办关于流式细胞术各种用途的系列研讨会。收购B.D.流式细胞仪Vantage SE II细胞仪将提供最先进的荧光激活细胞分选能力，既为中心的用户，也为圣地亚哥地区更大的研究社区。西德尼·金梅尔癌症中心已经确定了8个主要用户和6个次要用户，他们的研究项目需要或将通过使用这项技术得到显着增强。所有主要用户和大多数次要用户都有流式细胞术的经验，许多人经常使用邻近研究机构的细胞分选设施。大多数用户需要收集大量具有确定表型的纯化细胞用于各种体外和体内测定，其他用户需要筛选和选择成功转染的细胞系、细菌或从大样品中纯化非常罕见的细胞用于基因表达分析。FACS Vantage SE II仪器将非常适合这些需求，其具有多参数（多达8个荧光和2个光散射参数）检测能力，允许同时收集4个子集的4向分选，自动分选到多孔托盘中，分选速度为25，000个事件/秒，保留了出色的活力，功能和纯度。