Imaging ESCA Instrument

ESCA成像仪

• 批准号: 6581507
• 负责人: DAVID G CASTNER
• 金额: $ 49.95万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6581507
• 关键词: X ray spectrometry; biomedical equipment purchase; electron spin resonance spectroscopy; spectrometry

DESCRIPTION (provided by applicant): For biomedical systems, determining the chemical state (composition, molecular structure, and orientation) and distribution of biological moieties present on a surface is critical. Many of the important functions of cells and tissue depend on the arrangement of molecules at their surfaces. Also, a central goal of modern bioengineering is the development of biomaterial surfaces that direct the biological healing response. These novel surfaces are envisioned to have a well-defined array of recognition sites designed to interact specifically with cells. Thus, it is essential to develop surface analysis techniques capable of providing detailed surface chemical state information at high spatial resolutions. The Kratos AXIS ULTRA imaging electron spectroscopy for chemical analysis (ESCA) system requested in this application will provide essential, new capabilities for NIH-funded biomedical research since our 12 and 18 year old ESCA systems do not have imaging capabilities. Also, in the past 10 years instrumentation manufacturers have made significant advances in all areas of ESCA performance, so all types of ESCA experiments (composition, high resolution spectra, angle dependent, multi-sample and imaging analyses) will benefit significantly from the purchase of a new imaging ESCA system. The specific improvements provided by the new imaging AXIS ULTRA ESCA system over our current ESCA systems are: 1) increase of the spatial resolution for spot analysis by an order of magnitude; 2) provide a new chemical state imaging modality; 3) improve energy resolution by 20% in the spectroscopy mode; 4) increased sensitivity (lower detection limits); 5) more efficient and easier to use charge neutralization system; 6) computer controlled, multi-sample analysis for angle dependent experiments; and 7) decreased instrument downtime. Imaging ESCA provides quantitative surface compositional information about the distribution of both elements and functional groups at a spatial resolution of less than 10 microns. This information is essential for determining the structure-function relationship of the biomaterial surface and its biological activity. Relevant applications for imaging ESCA include biocompatibility of implants, biomolecule separations, cell culture, biosensors, bacterial-induced corrosion, ELISA assays, and DNA manipulations. Combining the capabilities of the proposed imaging ESCA system with existing imaging time-of-flight secondary ion mass spectrometry (ToF SIMS) and scanning probe microscopy (SPM) systems will provide a powerful set of complementary biomedical surface analysis techniques that will make a significant contribution to NIH-funded biomedical research projects.

描述（由申请人提供）： 对于生物医学系统，确定表面上存在的生物部分的化学状态（组成，分子结构和取向）和分布至关重要。细胞和组织的许多重要功能取决于其表面分子的排列。此外，现代生物工程的中心目标是开发指导生物愈合反应的生物材料表面。这些新的表面被设想为具有被设计为与细胞特异性相互作用的明确定义的识别位点阵列。因此，它是必不可少的，以发展表面分析技术，能够提供详细的表面化学状态信息，在高空间分辨率。本申请中要求的Kratos AXIS ULTRA成像电子能谱化学分析（ESCA）系统将为NIH资助的生物医学研究提供必要的新功能，因为我们12和18岁的ESCA系统没有成像功能。此外，在过去的10年中，仪器制造商在ESCA性能的所有领域都取得了重大进展，因此所有类型的ESCA实验（成分，高分辨率光谱，角度依赖，多样品和成像分析）都将从购买新的ESCA成像系统中受益匪浅。与我们现有的ESCA系统相比，新的成像AXIS ULTRA ESCA系统提供的具体改进是：1）将斑点分析的空间分辨率提高一个数量级; 2）提供新的化学状态成像模式; 3）在光谱模式下将能量分辨率提高20%; 4）增加灵敏度（较低的检测限）; 5）更有效和更容易使用的电荷中和系统; 6）用于角度依赖性实验的计算机控制的多样品分析;和7）减少的仪器停机时间。成像ESCA以小于10微米的空间分辨率提供关于元素和官能团分布的定量表面组成信息。这些信息对于确定生物材料表面的结构-功能关系及其生物活性是必不可少的。ESCA成像的相关应用包括植入物的生物相容性、生物分子分离、细胞培养、生物传感器、细菌诱导的腐蚀、ELISA测定和DNA操作。将所提出的成像ESCA系统的能力与现有的成像飞行时间二次离子质谱（ToF西姆斯）和扫描探针显微镜（SPM）系统相结合，将提供一套强大的互补生物医学表面分析技术，这将为NIH资助的生物医学研究项目做出重大贡献。