Mitochondria-based Biosensors in Microfluidic Devices

描述（由申请人提供）：本研究的目标是设计，制造和进行微流控系统与集成的基于生物传感器的试点研究。实现了一种基于吸附细胞色素C（cyt）释放和电化学检测的工作装置。c）由线粒体分泌。将从三个方面进行探讨。1-用于结合线粒体和细胞色素的自组装单层表面（SAM）化学。C将被识别。2-电化学研究将对细胞色素的氧化还原性质进行研究。c吸附在自组装膜上。3-微流体通道结合图案化的SAM用于结合线粒体和细胞色素。c和图案化的微电极。利用手头的工作微流体装置，将使用已知的细胞凋亡诱导剂和抑制剂来研究线粒体诱导的细胞凋亡。线粒体对细胞凋亡的错误调节与许多人类疾病有关：免疫缺陷、帕金森病、阿尔茨海默病和癌发生。拟议的研究不仅将为研究线粒体诱导的细胞凋亡事件提供灵敏的分析工具，而且还将产生用于高通量筛选启动和抑制线粒体分泌的凋亡信号的小分子的装置。

DESCRIPTION (provided by applicant): The goal of this research is to design, fabricate, and conduct pilot studies on microfluidic systems with integrated mitochondria-based biosensors. To realize a working device based on the release and electrochemical detection of adsorbed cytochrome c (cyt. c) secreted by mitochondria. Three aspects will be explored. 1-Self-assembled monolayer surface (SAM) chemistries for binding mitochondria and cyt. c will be identified. 2-Electrochemical studies will be carried out on the redox properties of cyt. c adsorbed onto SAMs. 3-Microfluidic channels incorporating patterned SAMs for binding mitochondria and cyt. c, and patterned microelectrodes, will be fabricated. With a working microfluidic device in-hand, mitochondria induced apoptosis will be studied using known inducers and inhibitors of apoptosis. The misregulation of apoptosis by mitochondria has been implicated in a number of human diseases: immunodeficiencies, Parkinson's disease, Alzheimer's disease, and carcinogenesis. The proposed research will not only provide a sensitive analytical tool for studying events in mitochondria induced cellular apoptosis, but will also produce a device for the high-throughput screening of small molecules that initiate and inhibit apoptotic signals secreted by mitochondria.