Mitochondria-based Biosensors in Microfluidic Devices

微流体装置中基于线粒体的生物传感器

• 批准号: 6820594
• 负责人: DOUGLAS Benjamin WEIBEL
• 金额: $ 4.73万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-29 至 2005-09-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6820594
• 关键词: apoptosis; biochemistry; biomedical equipment development; biophysics; biosensor device; biotechnology; cell membrane; cytochrome c; electrochemistry; fluidity; fluorescent dye /probe; high throughput technology; microelectrodes; mitochondria; molecular assembly /self assembly; nanotechnology; oxidation reduction reaction; postdoctoral investigator; surface plasmon resonance

DESCRIPTION (provided by applicant): The goal of this research is to design, fabricate, and conduct pilot studies on microfluidic systems with integrated mitochondria-based biosensors. To realize a working device based on the release and electrochemical detection of adsorbed cytochrome c (cyt. c) secreted by mitochondria. Three aspects will be explored. 1-Self-assembled monolayer surface (SAM) chemistries for binding mitochondria and cyt. c will be identified. 2-Electrochemical studies will be carried out on the redox properties of cyt. c adsorbed onto SAMs. 3-Microfluidic channels incorporating patterned SAMs for binding mitochondria and cyt. c, and patterned microelectrodes, will be fabricated. With a working microfluidic device in-hand, mitochondria induced apoptosis will be studied using known inducers and inhibitors of apoptosis. The misregulation of apoptosis by mitochondria has been implicated in a number of human diseases: immunodeficiencies, Parkinson's disease, Alzheimer's disease, and carcinogenesis. The proposed research will not only provide a sensitive analytical tool for studying events in mitochondria induced cellular apoptosis, but will also produce a device for the high-throughput screening of small molecules that initiate and inhibit apoptotic signals secreted by mitochondria.

描述（由申请人提供）：本研究的目标是设计，制造和进行集成线粒体生物传感器的微流体系统的试点研究。实现一种基于吸附细胞色素c （cyt）释放及电化学检测的工作装置。C)由线粒体分泌。将从三个方面进行探讨。自组装单层表面（SAM）化学结合线粒体和细胞。C将被识别。2 .对cyt的氧化还原性能进行电化学研究。c吸附在sam上。结合有图案的SAMs的微流控通道，用于结合线粒体和细胞。C和图案微电极将被制造出来。随着微流体装置的工作，线粒体诱导的细胞凋亡将使用已知的细胞凋亡诱导剂和抑制剂进行研究。线粒体对细胞凋亡的错误调控与许多人类疾病有关：免疫缺陷、帕金森病、阿尔茨海默病和癌变。本研究不仅将为研究线粒体诱导细胞凋亡事件提供一个灵敏的分析工具，而且还将产生一种高通量筛选启动和抑制线粒体分泌的凋亡信号的小分子的装置。

项目成果

Modeling the anodic half-cell of a low-temperature coal fuel cell.

对低温煤燃料电池的阳极半电池进行建模。

• DOI: 10.1002/anie.200501192
• 发表时间: 2005
• 期刊: Angewandte Chemie (International ed. in English)
• 作者: Weibel,DouglasB;Boulatov,Roman;Lee,Andrew;Ferrigno,Rosaria;Whitesides,GeorgeM
• 通讯作者: Whitesides,GeorgeM