DETERMINING THE DISSOCIATION CONSTANT OF A22 TO THE BACTERIAL CYTOSKELETON MREB

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 据报道，小分子苄基硫脲A22可与细菌细胞增殖素蛋白MreB结合，MreB是肌动蛋白同源物。 这种结合的确切性质仍然没有完全理解，也没有测量到A22的结合常数。 我们假设一系列的NMR实验将1）帮助确定A22是否与MreB相互作用，2）如果它确实相互作用，允许我们确定解离常数。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The small molecule, benzylthiourea A22 has been reported to bind to the bacterial cytoskelton protein MreB, which is an actin homolog. The exact nature of this binding is still not fully understood, and no binding constants for A22 have been measured. We hypothesize that a series of NMR experiements will 1) help establish whether A22 interacts with MreB, and 2) if it does interact, allow us to determine the dissociation constant.