Computational Studies of Protein Folding and Design

蛋白质折叠和设计的计算研究

• 批准号: 6710645
• 负责人: JEFFERY G SAVEN
• 金额: $ 24.4万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6710645
• 关键词: conformation; mathematical model; protein engineering; protein folding; protein sequence; statistics /biometry; structural biology

Protein folding is a crucial step in the expression of genetic information as functional molecules. Understanding this process, however, is obscured by the conformational complexity of proteins, our incomplete understanding of the determinants of folding, and the huge number of possible protein sequences. A statistical approach to understanding sequence-structure compatibility is being developed that can encompass all possible sequences. The method can assist researchers in (a) probing the sequence variability of protein structures, (b) focusing protein sequence libraries, and (c) designing sequences that fold to a predetermined structure. The theory will also be used to address the variability observed among naturally occurring protein sequences having a common structure. Lastly, information-based energy functions play a central role in protein design and structure prediction. A new class of such functions will be developed. The ability to understand the sequence variability of particular structures and to determine sequences that fold to desired conformations will advance our knowledge of genetic disorders, protein folding diseases and could lead to new types of protein based therapeutics.

蛋白质折叠是作为功能分子表达遗传信息的关键步骤。然而，由于蛋白质构象的复杂性、我们对折叠决定因素的不完全理解以及大量可能的蛋白质序列，对这一过程的理解变得模糊不清。目前正在开发一种统计方法来理解序列-结构相容性，它可以涵盖所有可能的序列。该方法可以帮助研究人员（a）探测蛋白质结构的序列变异性，（B）聚焦蛋白质序列文库，以及（c）设计折叠成预定结构的序列。该理论还将用于解决在具有共同结构的天然存在的蛋白质序列中观察到的变异性。最后，基于信息的能量函数在蛋白质设计和结构预测中起着核心作用。将开发一类新的此类函数。理解特定结构的序列变异性和确定折叠成所需构象的序列的能力将推进我们对遗传疾病、蛋白质折叠疾病的知识，并可能导致新型的基于蛋白质的疗法。