In toto Handheld Bioagent Detector by Digital Fluidics

Digital Fluidics 的手持式生物制剂检测器

• 批准号: 6895456
• 负责人: CHANG-JIN KIM
• 金额: $ 49.79万
• 依托单位: CORE MICROSOLUTIONS, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6895456
• 关键词: bacterial toxins; bioengineering /biomedical engineering; biohazard detection; biomedical equipment development; biosensor device; bioterrorism /chemical warfare; charge coupled device camera; dielectric property; diffusion; digital imaging; electrodes; fluid flow; microfluidics; microscopy; monitoring device; portable biomedical equipment; surface plasmon resonance

DESCRIPTION (provided by applicant): The design and evaluation of a handheld digital microfluidic (DMF) bioagent analyzer is proposed by Core Microsolutions (CMSS) and University of Michigan Professor K.-D. Lee (College of Pharmacy) for a two year Phase I research period in the NIH Small Business Biodefense Program. This proposed research will integrate: 1) Electrowetting on Dielectric (EWOD) droplet-based microfluidic handling technology pioneered by CMSS Chairman C.-J. Kim and 2) unique bacteria, toxin capture/sensing methods developed by Professor Lee, for a complete point-of-care diagnostic device that is built upon a programmable, low cost, and generic microfluidic chassis that can be mass-manufactured for wide distribution in clinics and hospitals. A digital microfluidics (DMF) device is capable of highly programmable and elegant droplet (approximately 250 nl volume) generation, transport and splitting on a simple electrode array and is accomplished without the complex and costly micropump, microvalve, microchannel structures typically needed for lab-on-chip sensing devices. DMF handling also lowers reagent, sample consumption and opens up the possibility of signal amplification when a steady stream of droplets is directed to a single sensing site to increase target capture. Bacteria and toxin capture and sensing techniques developed by Professor Lee can be integrated directly on the EWOD electrode array and are hypothesized to provide bacteria and toxin detection with simple resistance or capacitance measurements. This Phase I feasibility of a DMF biosensor cartridge will be determined by evaluating limits of detection (LOD), signal/noise ratio, and dynamic range of several sample solutions with varying concentrations of bacteria and toxin targets. Precision and failure points of EWOD droplet handling will also be characterized. To accelerate the transition to Phase II commercial research, deliverables will include: a DMF biosensor cartridge, an optimized (2nd gen.) DMF microfluidics cartridge and an integrated EWOD controls module.

描述（由申请人提供）：由核心微量液体（CMSS）和密歇根大学教授K.-D.提出了手持数字微流体（DMF）生物代理分析仪的设计和评估。 Lee（药学院）在NIH小型企业Biodefense计划的两年I阶段研究期间。这项拟议的研究将整合：1）基于介电（EWOD）基于液滴的微流体处理技术，由CMSS董事长C.-J.率先使用的微流体处理技术。 Kim和2）由Lee教授开发的独特细菌，毒素捕获/感应方法，为完整的护理点诊断设备而建立，该设备建立在可编程，低成本和通用的微流体底盘上，可以大规模制造，以在临床和医院中进行广泛的分布。数字微流体（DMF）设备能够在简单的电极阵列上产生高度可编程和优雅的液滴（约250 nL量）的生成，运输和分裂，并且在没有复杂且昂贵的微型泵，微羊角，微通道结构的情况下可以完成，而实验室式接触式接触式感应设备通常需要。 DMF处理还可以降低试剂，样品消耗并打开信号放大的可能性，当时稳定的液滴流向单个感应位点以增加目标捕获。 Lee教授开发的细菌和毒素捕获和感测技术可以直接集成在EWOD电极阵列上，并假设以简单的耐药性或电容测量为细菌和毒素检测。 DMF生物传感器弹药筒的可行性将通过评估检测限（LOD），信号/噪声比以及几种具有不同浓度的细菌和毒素靶标的样品溶液的动态范围来确定。 EWOD液滴处理的精度和故障点也将被表征。为了加速向II期商业研究的过渡，可交付成果包括：DMF生物传感器墨盒，优化的（第二代）DMF微流体墨盒和集成的EWOD控制模块。