Polymer Membrane Ion/Polyion Sensors: New Frontiers

描述（由申请人提供）：继续开发、研究和 新型聚离子和阴离子选择性聚合物的生物分析应用 提出了基于膜/膜的电化学和光学传感器。研究 在这个长期项目的下一阶段， 在最近项目期间取得的重大发现/进展 与电位聚离子传感和新化学物质有关 阴离子选择性传感器这些措施包括大幅降低检测 对生物医学上重要的聚离子的限制（例如，肝素、鱼精蛋白等） 通过使用新颖的旋转电位膜电极结构， 和2）揭示了独特的氢氧离子桥连的二聚体/单体平衡， 可以存在某些金属卟啉时，作为离子载体在有机 用于设计阴离子传感电极的聚合物膜。今后的努力将 包括聚离子和阴离子的基础和应用研究 感应区。聚离子传感器工作的具体目标包括： 理解性质（大小、化学计量等）的 在聚合物膜内形成的离子交换剂/聚离子复合物， 制造这样的装置; B）证明 新的、更灵敏的旋转聚离子传感膜电极 配置; c）调查聚离子传感器的使用（包括旋转 排列）来检测聚阳离子树枝状聚合物及其与DNA的相互作用; 和d）进一步开发一种全新的、快速的均质 一种用于检测微小、临床和环境中 使用聚离子作为标记的重要分析物。在阴离子传感器领域， 我们的努力将集中在确定哪些金属卟啉可以自发地 在聚合物膜内形成氢氧根离子桥接的二聚体结构，和 确定这些反应如何影响阴离子的响应特性 用这种离子载体配制的选择性电极。此外，研究将 利用这种新的化学方法来设计全新的聚合物 用于阴离子和中性物质（胺、气体等）的膜基光学传感器 基于这种配位物质破坏金属卟啉二聚体的能力 转化为有机膜内的单体，产生了很大的位移， Soret乐队基于阴离子表面活性剂的电化学阴离子传感器的研究 提出了一种新型的亲脂树枝状阴离子交换剂结构。它 预计这项研究将继续提供分析 社区拥有大量新的和/或改进的化学传感器， 新的基于传感器的方法，将有直接的应用程序作为工具， 基础研究以及现代临床和环境试验 仪器仪表

DESCRIPTION (provided by applicant): The continued development, study, and bioanalytical applications of novel polyion and anion selective polymer membrane/film-based electrochemical and optical sensors are proposed. Research during the next phase of this long-term project will build upon several significant discoveries/advances made during the most recent project period relating to potentiometric polyion sensing and new chemistries for anion-selective sensors. These include dramatically lowering the detection limits toward biomedically important polyions (e.g., heparin, protamine, etc.) via use of a novel rotating potentiometric membrane electrode configuration, and 2) uncovering a unique hydroxide ion bridged dimer/monomer equilibrium that can exist for certain metalloporphyrins when used as ionophores in organic polymer films for devising anion sensing electrodes. Future efforts will include both fundamental and applied studies in both the polyion and anion sensing areas. Specific goals for the polyion sensor efforts include: a) better understanding the nature (size, stoichiometry, etc.) of the ion-exchanger/polyion complexes that form within the polymer membranes used to fabricate such devices; b) demonstrating broader bioanalytical applications of the new, more sensitive rotating polyion sensing membrane electrode configuration; c) investigating the use of polyion sensors (including rotating arrangement) to detect polycationic dendrimers and their interaction with DNA; and d) further development of a completely new and rapid homogeneous immunoassay method for detecting of small, clinically and environmentally important analytes using polyions as labels. In the area of anion sensors, efforts will focus on establishing which metalloporphyrins can spontaneously form hydroxide ion bridged dimer structures within polymeric films, and determining how these reactions affect the response properties of anion selective electrodes formulated with such ionophores. In addition, studies will be undertaken to utilize this novel chemistry to devise completely new polymer film-based optical sensors for anions and neutral species (amines, gases, etc.) based on the ability of such ligating species to break metalloporphyrins dimers into monomers within the organic films, yielding a large shift in the lambdamax of the Soret band. Investigations of electrochemical anion sensors based on a new type of lipophilic dendntic anion-exchanger structure are also proposed. It is anticipated that this research will continue to provide the analytical community with a wide array of new and/or improved chemical sensors as well as novel sensor-based methods that will have immediate applications as tools for basic research and within modem clinical and environmental test instrumentation.