Core--Animal Physiology

动物生理学核心将在每个项目的许多研究中发挥重要作用。这一核心支持的程序包括对大鼠和小鼠的脑室和特定的下丘脑核团进行插管，颈静脉插管采血，在研究结束时收集脑和其他组织，并准备这些材料用于存储或进一步分析，代谢研究包括通过热量测量的氧气消耗和呼吸商，体力活动测量，进食模式分析，以及通过磁共振光谱分析身体成分。最后，该核心将支持每个项目常见的特定生化或分子分析的性能，包括大鼠和小鼠下丘脑rnRNA物种的实时聚合酶链式反应，以及用于检测特定信号分子(例如，磷酸化ACC和磷酸化PKB)的蛋白质印迹分析。动物 生理学核心人员已经拥有了执行本提案中所包括的所有实验所需的专业知识和资源，现有的方案将确保有效地实现这一核心的目标。

The Animal Physiology Core will play an essential role in many studies in each of the projects. Procedures supported by this core include cannulation of brain ventricles and specific hypothalamic nuclei of rats and mice, jugular vein catheterization for blood sampling, collection of brain and other tissues at that time of study termination and preparation of those materials for storage or for further analysis, metabolic studies including oxygen consumption and respiratory quotient by calorimetry, physical activity measurements, meal pattern analysis, and body composition analysis by magnetic resonance spectroscopy. Finally, this core will support the performance of specific biochemical or molecular assays that are common to each project including real time PCR ofhypothalamic rnRNA species in rats and mice and western blot analysis for detection of specific signaling molecules (e.g., phospho-ACC and phospho-PKB). Animal Physiology Core personnel already have the expertise and access to resources necessary to perform all of the experiments included in this proposal, and existing protocols will ensure that the goals of this core are efficiently met.