CORE C--ADMINISTRATIVE

核心C——行政

• 批准号: 6859761
• 负责人: THOMAS Scott EDGINGTON
• 金额: $ 24.46万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6859761
• 关键词: CHO cells; biomedical facility; biotechnology; hybridomas; immunologic substance development /preparation; peptide chemical synthesis; protein purification; recombinant proteins; tissue /cell preparation; transfection

This core is a necessary and integral component of this Program Project. It provides highly experienced technical expertise for the projects needed to meet the goals of the Program. It provides for the production and purification of the many recombinant proteins, wild-type or modified, for the projects. This demands production by bacterial hosts as well as eukaryotic hosts such as CHO-K1 and variant cells that have been engineered for proper post-translational modification of proteins such as proper glycosylation, gamma carboxylation, proteolytic processing, analysis for purity and functional integrity. This core provides for gene transfer to cells cloning and expansion of these clones of modified cells. It provides for antisera to new proteins or specific for modifications of proteins. Also, it is very expert in hybridoma generation for monoclonal antibody production, selection, and establishing long-term high affinity and highly specific monoclonal antibody reagents for the projects where such reagents do not otherwise exist. The expertise is shared with investigators who are newly involved in the various technologies. The core personnel assist the various investigators in conduct of more sophisticated electrophoretic techniques and processing for mass spectroscopy. They supervise the proper use of common shared equipment. The core personnel are among the most expert personnel in sterile technique, cell culture, monoclonal antibody production, safety management, and recombinant protein production in the Institute.

这一核心是本计划项目不可或缺的组成部分。它提供了高度的 为实现方案目标所需的项目提供了经验丰富的技术专长。它 用于生产和纯化许多重组蛋白，野生型或 已修改，用于项目。这需要细菌宿主以及真核宿主(如CHO-K1和变异细胞)的生产，这些细胞已经被设计为适当的翻译后 蛋白质的修饰，如适当的糖基化、伽玛羧化、蛋白水解物 加工、纯度和功能完整性分析。这一核心为基因转移到细胞、克隆和扩增这些修饰细胞的克隆提供了条件。它提供针对新蛋白质的抗血清或蛋白质修饰的特异性。此外，它在杂交瘤的产生、筛选和建立长期高亲和力和高亲和力的杂交瘤方面也是非常专业的。 专属的单抗试剂，用于不存在此类试剂的项目。这些专业知识与新参与各种技术的调查人员分享。核心人员协助不同的研究人员进行更复杂的电泳技术和质谱学处理。他们监督公共共享设备的正确使用。核心人员在无菌技术、细胞培养、单抗生产、安全管理和重组蛋白生产方面是研究所最专业的人员之一。