CORE C--ADMINISTRATIVE

核心C--行政

• 批准号: 7237994
• 负责人: THOMAS Scott EDGINGTON
• 金额: $ 21.29万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7237994
• 关键词: CHO cells; biomedical facility; biotechnology; hybridomas; immunologic substance development /preparation; peptide chemical synthesis; protein purification; recombinant proteins; tissue /cell preparation; transfection

This core is a necessary and integral component of this Program Project. It provides highly experienced technical expertise for the projects needed to meet the goals of the Program. It provides for the production and purification of the many recombinant proteins, wild-type or modified, for the projects. This demands production by bacterial hosts as well as eukaryotic hosts such as CHO-K1 and variant cells that have been engineered for proper post-translational modification of proteins such as proper glycosylation, gamma carboxylation, proteolytic processing, analysis for purity and functional integrity. This core provides for gene transfer to cells cloning and expansion of these clones of modified cells. It provides for antisera to new proteins or specific for modifications of proteins. Also, it is very expert in hybridoma generation for monoclonal antibody production, selection, and establishing long-term high affinity and highly specific monoclonal antibody reagents for the projects where such reagents do not otherwise exist. The expertise is shared with investigators who are newly involved in the various technologies. The core personnel assist the various investigators in conduct of more sophisticated electrophoretic techniques and processing for mass spectroscopy. They supervise the proper use of common shared equipment. The core personnel are among the most expert personnel in sterile technique, cell culture, monoclonal antibody production, safety management, and recombinant protein production in the Institute.

这个核心是这个项目的必要和不可分割的组成部分。它提供了