CORE--LABORATORY

实验室核心为各个项目的重组蛋白生产、蛋白纯化和这些试剂的标准质量控制提供集中资源。这是一个现有的核心单元，已经建立了用于重组生产维生素K依赖性凝血因子VIIa和X的哺乳动物细胞系的转染、选择和大规模生长的所有方面。可溶性TF和位点特异性突变体在E. coli或S.酿酒酵母是建立的常规程序，包括重折叠和纯化至均一，以成功结晶这些蛋白质[1，2]。血浆蛋白纯化以及抗体生产和片段化的方案已经到位。因此，该中心集中生产各项目所用的基本试剂。

The laboratory core provides centralized resources for recombinant protein production, protein purification and standard quality control of these regents for the individual projects. This is an existing core unit that has established all aspects of transfection, selection and large scale growth of mammalian cell lines for the recombinant production of Vit.K dependent coagulation factors VIIa and X. Expression of soluble TF and site specific mutants in E. coli or S. cerevisiae are established routine procedures, including refolding and purification to homogeneity for successful crystallization of these proteins [1,2]. Protocols for the purification of plasma proteins, as well as, for the antibody production and fragmentation are in place. This core thus centralizes the production of essential reagents used by the various projects.